摘要
目的:研究来曲唑诱导的多囊卵巢综合征(PCOS)模型大鼠卵巢组织中促卵泡刺激素受体(FSHR)基因的表达,为该模型的应用提供一定的理论依据。方法:40只雌性SD大鼠随机分为模型组和对照组,每组20只,模型组应用来曲唑诱导大鼠PCOS模型,对照组不作任何处理。应用放射免疫法测定大鼠血清性激素水平;HE染色观察卵巢组织学变化,Real-time-PCR、免疫组织化学和Western blotting法检测FSHR基因在卵巢组织中的表达。结果:与对照组比较,模型组血清睾酮(T)、黄体生成素(LH)、卵泡刺激素(FSH)浓度显著增高(P<0.05),雌二醇(E2)、孕酮(P)浓度降低(P<0.05)。卵巢质量两组间比较差异无显著性(P>0.05)。与对照组比较,模型组可见囊状扩张卵泡明显增多,卵巢白膜增厚,黄体数量明显减少。模型组卵巢组织中FSHR mRNA及蛋白表达水平均显著高于对照组(P<0.05)。结论:来曲唑诱导的PCOS大鼠卵巢组织中FSHR基因表达水平与人类PCOS患者相似,该模型是研究PCOS病理机制的一种理想的动物模型。
Objective To investigate the expressions of FSH receptor mRNA and protein in ovary tissue in rats with letrozole-induced polycystic ovary syndrome（PCOS）,and to provide experimental data for the model application. Methods Forty rats were randomly divided into two groups（n=20）,in PCOS model group letrozole was administered once daily during 21 d,and in control group without any treatment.The gonadal hormone concentrations in serum were determined by radioimmunoassay,the histologic changes in ovaries were observed by HE staining,the expression of FSH receptor gene in ovary tissue was detected by realtime -PCR,Western blotting and immunohistochemistry.Results Compared with control group,estradiol（E2） and progesterone in model group showed a considerable reduction（P〈0.05）,the serum testosterone （T） and follicle-stimulating hormone （FSH） and luteinizing hormone（LH） levels in model group were markedly increased than those in control group（P〈0.05）.The ovarian weights of the two groups had no significant difference（P〉0.05）.Compared with control group,the ovaries from model group showed high incidence of subcapsular ovarian cyst and capsular thickening and decreased number of corpora lutea.The expressions of FSH receptor mRNA and protein were significantly higher in model group than those in control group（P〈0.05）.Conclusion The expression of FSH receptor gene in letrozole-induced polycystic ovaries is similar with that of PCOS women,the rat model is proved to be an ideal PCOS animal model to study the pathophysiology of PCOS.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2009年第6期1048-1051,I0012,共5页
Journal of Jilin University：Medicine Edition
基金
广东省科技厅自然科学基金资助课题(8152402301000007)
