摘要
目的探讨GATA-4在多囊卵巢综合征(PCOS)发病机制中的作用,并为来曲唑诱导的PCOS模型的应用提供一定的理论依据。方法 40只雌性SD大鼠随机分为模型组和对照组,模型组来曲唑1mg/(kg.d)溶于10g/L羧甲基纤维素(CMC)中,连续灌服21d;对照组给予同体积的溶剂(CMC)灌服。放射免疫法测定大鼠血清性激素水平;HE染色观察卵巢组织学变化;免疫组织化学、实时定量PCR和免疫印迹法检测GATA-4在卵巢组织中的表达。结果模型组血清睾酮(T)、促黄体生成素(LH)、促卵泡刺激素(FSH)浓度显著增高,雌二醇(E2)、孕酮(P)浓度降低。与对照组比较,模型组可见囊状扩张卵泡明显增多,卵巢白膜增厚,黄体数量明显减少。模型组卵巢窦前卵泡和窦状卵泡颗粒细胞GATA-4表达显著增强,卵巢组织中GATA-4mRNA及蛋白表达水平均显著高于对照组(P<0.05)。结论卵泡颗粒细胞GATA-4表达异常增高与多囊卵巢综合征的发生密切相关。来曲唑诱导的PCOS动物模型是研究PCOS病理机制的一种理想的动物模型。
Objective To investigate the role of GATA-4 in the pathogenesis of polycystic ovary syndrome （PCOS）, and to provide some experimental data for the application of the rat model induced by letrozole. Methods Forty female rats were randomly divided into two groups, PCOS model group and control group. The rats in model group were administered letrozole with the dosage of 1 rag/（ kg· d） dissolved in 10g/L carboxymethyl cellulose （CMC） by P. O./Q. D. for 21 days, and rats in the control group were administered with equal dosage and volume of CMC by P. O/Q. D. for 21 days. Gonadal hormone concentrations in serum were determined by radioimmunoassay; the histological changes in ovarian tissues were observed with HE staining, and the expression of GATA-4 in ovary tissue was detected by immunohistochemistry, real-time PCR and Western blotting. Results With the comparison of two groups, the concentrations of estradiol and progesterone were found considerably reduced in the control group （P 〈 0.05）, while the serum testosterone, follicle-stimulating hormone （FSH） and luteinizing hormone（LH） levels were found increased markedly in the model group （P 〈 0.05 ）. The ovaries in the model group showed high incidence of subcapsular ovarian cyst and capsular thickening and decreased number of corpora lutea comparing to those in the control group. The expression of GATA-4 in preantral follicle and antral follicle granulosa cells in the model group was significantly higher than those in the control group. With the observation on and comparison of staining densities, the mRNA transcription and protein depression of GATA-4 were significantly stronger in the model group than those in the control group （ P 〈 0.05 ）. Conclusion The extra higher expression of GATA-4 in follicle granulosa cells may be significantly correlated with the pathophysiology mechanism of PCOS. The letrozole-induced PCOS rat model is an ideal PCOS animal model for studying the pathogenesis of PCOS.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第5期728-732,共5页
Acta Anatomica Sinica
