摘要
目的研究某药物对铅染毒小鼠抗氧化作用的影响。方法用醋酸铅水溶液为小鼠腹腔注射染毒。然后将染毒小鼠分成治疗1组、治疗2组和模型组,另设正常组。治疗1组、治疗2组用不同剂量的某药物灌服治疗。实验结束,从眼球取血,分离血清。处死动物后,取大脑制成10%脑匀浆。测定各组血、脑超氧化物歧化酶(SOD)、脑谷胱甘肽(GSH)、乙酰胆碱酯酶(TCHE)等生化指标。结果血清总SOD活力模型组明显低于其他各组,差异有统计学意义(P<0.01);脑SOD活力正常组和治疗1组明显高于其他两组,差异有统计学意义(P<0.05,P<0.01);脑GSH含量模型组明显高于其他组,差异有统计学意义(P<0.01);脑TCHE活力治疗1组明显低于正常组和治疗2组,差异有统计学意义(P<0.01);脑铅含量模型组明显高于其他组,差异有统计学意义(P<0.01,P<0.05);血清丙氨酸氨基转移酶(ALT)模型组明显高于其他组,差异有统计学意义(P<0.01);血清尿素各组间比较,差异无统计学意义。结论治疗1组和治疗2组的血清SOD、ALT、脑SOD,脑铅和GSH均接近正常组,与不治疗的模型组比较,差异有统计学意义,治疗后小鼠抗氧化能力和肝功能均得到保护。
Objective To study the antioxidant effect of a pharmaceutical on lead poisoned mice. Methods Lead acetate solution was administered into mice by ip injection. Poisoned mice were divided as treatment group 1, treatment group 2 and model group. A control group was set. Treatment group 1 and 2 were instilled with different dosages of the pharmaceutical. After the experiments blood samples were collected from the eyeballs and serum samples were separated. After the mice were sacrificed, brains were collected and made into 10% homogenous paste. Bio-indicators such as SOD, glutathione （GSH）and acetyl cholinesterase（TCHE）in blood and brain of every group were determined. Results Overall SOD in the serum of model group was obviously lower than those of other groups（P〈0.01）. The activities of SOD in brain of the control group and treated group 1 were obviously higher than those of other two group（P〈0.05, P〈 0.01）. The content of GSH in the brain of model group was obviously higher than those of other groups（P〈 0.01）. The activity of TCHE in brains of treatment group 1 was obviously lower than those of the control group and treatment group 2（P〈0.01）. The contents of lead in the brain and amino-alanine（ALT）in the serum of model group were obviously higher than those of other groups（P〈0.01）. No significant difference existed in the urea contents in blood serum of various groups. Conclusions Serum SOD and ALT, and brain SOD, lead and GSH in treatment groups 1 and 2 approach to normal. In comparison with the model group the difference is significant. The results indicate that after treatment both the anti-oxidation ability and liver function of mice are protected.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2009年第6期344-347,共4页
Industrial Health and Occupational Diseases
关键词
铅染毒
小白鼠
抗氧化功能
Lead exposure
Mice
Anti-oxidation ability
