摘要
目的对比研究乳腺癌中免疫组化法(IHC)检测Her-2蛋白表达和荧光原位杂交法(FISH)检测Her-2基因扩增检测结果的差异性和相关性,并评估其临床应用价值。方法应用IHC和FISH技术检测50例乳腺癌组织中Her-2蛋白表达及Her-2基因扩增状况,并进行对比分析。结果50例乳腺癌中,8例Her-2蛋白表达IHC(+++)的病例中有7例Her-2基因扩增(87.5%),7例IHC(++)的病例有6例Her-2基因扩增(85.71%),3例IHC(+)的病例有2例Her-2基因扩增(66.67%),而在32例IHC(0)的乳腺癌中仍出现2例Her-2基因扩增(6.25%)。IHC和FISH两种方法检测结果的总符合率为88.0%,两者呈显著相关(P<0.001)。结论IHC是初步筛查乳腺癌Her-2状态的首选方法,Her-2蛋白高强度表达IHC(+++)与基因扩增有较好的一致性,而弱中强度表达IHC(+～++)者有必要进一步行FISH法检测基因状态来指导临床的诊疗。
Objective To investigate the difference and the relation of immunohistochemistry（IHC） for detecting the expression of Her- 2 protein and fluorescence in situ hybridization（FISH） for detecting Her-2 gene amplification in breast cancer,and to evaluate the value of the clinical application. Methods The expression of Her-2 protein and Hero2 gene amplification in 50 cases of breast cancer were detected and compared by IHC and FISH methods,respectively. Results Seven cases of Her-2 gene amplification were found in 8 cases of IHC（ ＋ ＋ ＋ ） （7/8,87.5%） ,6 cases of Her-2 gene amplification were detected in 7 cases of IHC（ ＋ ＋ ） （6/7,85.71%） ,2 cases of Her-2 gene amplification were observed in 3 cases of IHC （ ＋ ） （ 2/3,66.67 % ）, and 2 cases of gene amplification existed in 32 cases of IHC（0） （2/32,6.25%）. The accordance rate of IHC and FISH was 88.0% ,and the results by two methods were significandy related（P 〈 0. 001 ）. Conclusion IHC is a preferred method for detecting the Her-2 in breast cancer. The strongly positive expression （ ＋ ＋ ＋ ） of Her-2 protein tested by IHC is greatly consistent with Her-2 gene amplification by FISH, but Her-2 gene amplification should be further detected in patients with Her-2 positive expression（ ＋ - ＋ ＋ ）by FISH in order to guide the clinical diagnosis and treatment.
出处
《山西医科大学学报》
CAS
2009年第11期972-975,1056,共5页
Journal of Shanxi Medical University
基金
卫生部科研基金资助项目(WKJ2007-3-001)
