摘要
目的了解乳腺癌组织中人表皮生长因子受体2(HER-2)基因扩增与Her-2/neu蛋白表达的一致性与相关性。方法采用荧光原位杂交(FISH)法及免疫组化(IHC)法分别检测349例浸润性乳腺癌患者的HER-2基因扩增与蛋白表达情况,分析二者检测结果的一致性与相关性。结果 FISH与IHC符合率为55.3%,结果存在一致性(Kappa=0.189,P=0.000),呈正相关(r=0.434,P=0.000)。结论 FISH与IHC两种检测结果存在一致性,但IHC(+^+++)患者均应以FISH检测作为评价HER-2基因是否扩增的标准方法。
Objective To investigate the correlation between human epidermal growth factor receptor 2（HER-2） gene amplification and Her-2/neu protein expression in invasive breast cancer patients. Methods Using fluorescence in situ hybridization （FISH） and immunohistochemistry（IHC） method, the HER-2 gene amplification and Her-2/neu protein expression status were detected, respectively, and its concordance and correlation in a total of 164 samples of invasive breast cancer tissues. Results The overall coincidence rate of FISH and IHC was 55.3% （Kappa=0.189, P-0.000）, with positive correlation （r=0.434, P=0.000） between them. Conclusion There is a concordance between the Her-2/neu protein expression and its gene amplification. HER-2 gene amplification status should be further detected by FISH in patients with HER-2 protein expression （＋ ~ ＋＋＋）.
出处
《中国医药科学》
2013年第20期16-18,共3页
China Medicine And Pharmacy
基金
泸州医学院博士启动基金(2011204)
关键词
乳腺癌
人表皮生长因子受体-2
荧光原位杂交
免疫组织化学
Breast cancer
Human epidermal growth factor receptor 2 （HER-2）
Fluorescence in situ hybridization（FISH）
Immunohistochemistry （IHC）
