摘要
目的探讨次声作用对成年大鼠脑室下区(SVZ)神经前体细胞增殖的影响。方法成年雄性Sprague-Dawley大鼠(n=24)随机分为正常对照组、对照组和次声组,次声组动物置于次声压力仓(16Hz、130dB)内连续作用7d(2h/d)。照射结束后,分别于3d、7d、10d和14d处死。处死前各组大鼠腹腔注射5-溴脱氧尿嘧啶尿苷(BrdU)3次,以BrdU免疫组织化学法检测各组SVZ内增殖细胞数目的变化。结果次声照射结束后3d,次声处理组动物SVZ区的BrdU阳性细胞数目较对照组显著减少(P<0.01),7d时阳性细胞数继续降低(P<0.01),但10d时开始逐渐恢复,14d时达到正常水平(P>0.05)。结论16Hz、130dB次声可抑制成年大鼠SVZ神经前体细胞增殖。
Objective To study the effect of infrasound on the proliferation of neural precursors in the subventricular zone （SVZ） of adult rats. Methods A total of 24 male SD rats were randoudy divided into normal control group, control group and infrasound group. The rats of infrasound group were exposed to infrasound of 16 Hz at 130 dB for 2 h/d in 7 consecutive days. Before the rats were sacrificed at 3 d ,7 d, 10 d and 14 d after infrasound exposure, 5-bromodeoxyuridine （BrdU） were given intraperitoneally for three times to label the Sphase cells, which were detected by anti-BrdU immunostaining. Results BrdU-positive cells were significantly decreased in the SVZ at 3 d after infrasound exposure and continued to reduce at 7 d, compared with those of the control group at 3 d and 7 d, respectively （P 〈 0.01 ）. However, 10 d after infrasound exposure, a large number of BrdU-positive cells reappeared in the SVZ,and the number of BrdU-positive cells reached the nomrJ1 level at 14 d （P 〉0. 05）. Conclusion Infrasound of 16 Hz at 130 dB inhibits the proliferation of neural precursors in the SVZ of adult rats.
出处
《中华神经外科疾病研究杂志》
CAS
2009年第5期424-427,共4页
Chinese Journal of Neurosurgical Disease Research
基金
国家自然科学基金资助项目(30570443)
全军医药卫生专项课题基金资助项目(062040)
关键词
次声
脑室下区
细胞增殖
神经发生
大鼠
Infrasound
Subventlicular zone
Cell proliferation
Neurogenesis
Rats
