摘要
目的:一定声压级水平的次声作用可引起组织器官的损伤。研究16Hz,90,110和130dB的次声暴露对人脐血管内皮细胞ECV-304)内钙离(子浓度的影响,探讨次声对细胞损伤作用的机制。方法:实验于2003-10/2004-06在西安第四军医大学附属一院物理医学与康复医学科次声实验室及电镜中心完成。将ECV-304接种于细胞爬片上,并分为对照组、假暴露组和16Hz,90,110和130dB的次声暴露组。对实验组的细胞作2h的次声暴露,采用钙离子荧光探针结合激光扫描共聚焦显微镜观察次声暴露后细胞内钙离子浓度的变化。结果:经次声暴露2h后,90dB次声暴露组(427.4±57.1)、110dB次声暴露组(489.1±63.7)和130dB次声暴露组(531.3±61.9)与对照组比较,差异均有显著性意义t=8.6,6.9,8.3,P<0.01)。130dB组(的细胞内钙离子浓度明显高于90dB组(t=3.0,P<0.05)。结论:不同声压级水平的16Hz次声暴露可导致血管内皮细胞内钙离子浓度的不同程度增高,从而引起机体血管内皮细胞的损伤性改变,且钙离子浓度的增高与声压级水平相关。
AIM:Infrasound of certain sound pressure level can cause injury of tissue and organ. This paper aims to study the effects on concentration of calcium ion(Ca2+ ) in human umbilical vein endothelial cells(EVC 304 cells) exposed by 16 Hz infrasound with intensity output of 90,110 and 130 dB,and investigate the mechanisms of infrasound in cell injury.METHODS:The experiment was carried out in the Department of Physical Medicine and Rehabilitation and Infrasound Laboratory of the First Affiliated Hospital of the Fourth Military Medical University of Chinese PLA from October 2003 to June 2004.After the ECV 304 cells were inoculated to coverglass,they were divided into control group,sham exposed group,16 Hz infrasound with intensity output of 90,110 and 130 dB exposure groups.Cells in the experimental groups were exposed to infrasound for 2 hours.The changes of Ca2+ concentration after exposure to infrasound were observed with Ca2+ Fluo 3/AM fluorescence technique and Laser scanning confocal microscope. RESULTS:After exposure to infrasound for 2 hours,the Ca2+ concentrations in the 90,110 and 130 dB infrasound exposure groups(427.4 ± 57.1,489.1± 63.7 and 531.3± 61.9) were significantly different from that in the control group(t=8.6,6.9,8.3, P< 0.01),and it was obviously higher in the 130 dB group than in the 90 dB group (t=3.0, P< 0.05). CONCLUSION:The 16 Hz infrasound exposure of different sound pressure levels can lead to different increase of intracellular Ca2+ concentration in ECV 304 cells, and the increase of Ca2+ concentration is related to sound pressure level, which has provide experimental data for the defensive mechanism of acoustic injury of tissue and organ.
出处
《中国临床康复》
CSCD
北大核心
2005年第11期48-49,共2页
Chinese Journal of Clinical Rehabilitation
