摘要
目的综述药用植物次生代谢酶基因克隆可采用的方法策略,为其基因克隆提供参考。方法查阅相关文献进行总结和归纳。结果阐述了用于克隆药用植物次生代谢酶基因的功能克隆方法、表型克隆方法(DD-PCR、cDNA–AFLP、SSH)、转座子标签克隆方法及图位克隆方法。结论适用于药用植物次生代谢相关酶基因克隆的技术方法有很多种,研究者可根据研究基础、目的和实验条件等进行选择。
OBJECTIVE Reviewing gene cloning methods and strategies that can be used in medicinal plant secondary metabolism, to provide selection references. METHODS Searching and summarizing literatures on gene cloning of plants, especially of medicinal plants. RESULTS Gene cloning methods used in medicinal plant secondary metabolism, such as functional cloning method, phenotype cloning methods, transposon tagging and map-based cloning method were elaborated. CONCLUSION There are many methods used to clone genes in medicinal plant secondary metabolism, researchers should choose the suitable one with different basis, purposes and experiment condition.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第10期805-809,共5页
Chinese Journal of Modern Applied Pharmacy
基金
国家科技支撑计划重点项目(2006BAI09B01)
博士后科学基金资助(20070410615)
中央级院所长科研基金项目(YZ-1-10)
关键词
药用植物
次生代谢
基因克隆
medicinal plants
secondary metabolism
gene cloning
