摘要
目的:采用液相色谱-质谱联用方法快速分析双黄连粉针(金银花、黄芩和连翘)中的成分。方法:采用Zorbax C18柱,以乙腈-水溶液梯度洗脱,电喷雾质谱在线检测,全离子扫描模式扫描,对每一组分峰进行质谱分析。结果:通过质谱分析及文献对照,从双黄连粉针图谱中共准确推断18个峰的分子量,鉴定出9个化合物,分别为奎尼酸、咖啡酸、三种单咖啡酰奎尼酸、异绿原酸、连翘苷、连翘酯苷、黄芩苷。结论:首次从双黄连粉针剂中检测出奎尼酸,液相色谱-质谱联用技术是快速分析中药化学组分的有效手段。
AIM ： To analyse the main chemical constituents of Shuanghuanglian Injection Power （ Flos lonicerae japanicae, Radix scutellariae and Fructus forsythiae） by high performance liquid chromatography-electrospray ionization-mass/mass spectrometry. METHODS： The sample solution was analysed by a Zorbax C18 column with a gradient mobile phase comprised of acetonitrile and water. Electrospray ionization mass spectrometry detector was used, full-scan detection mode was evaluated for the identification of the liquid chromatography peaks. RESULTS ： To analyse the mass spectrum of every liquid chromatography peaks, we identified 18 molecular mass from the ion chromatogram of Shuanghuanglian Injection Powder, 9 compounds were identified, including quinic acid , caffeic acid, three monocaffeoylquinic acids, dicaffeoylquinic acid,forsythin, forsythoside and baicalin. CONCLUSION： Quinic acid is identified from Shuanghuanglian Injection Power for the first time. High performance liquid chromatography-mass/mass spectrometry is emerging as an important tool in traditional Chinese medicine.
出处
《中成药》
CAS
CSCD
北大核心
2009年第9期1402-1404,共3页
Chinese Traditional Patent Medicine
