摘要
目的优选黄芪总黄酮的提取工艺。方法用正交设计法探讨了影响纤维素酶法提取黄芪中总黄酮的主要因素,并确定了最佳提取工艺。结果纤维素酶法提取黄芪总黄酮的最佳工艺条件为:酶解时间为120 m in,酶解温度为30℃,酶量为8 mg,pH值为4.5。结论此工艺简单可行,是一条提取黄芪总黄酮类物质的有效途径。
Objective To opmtimize the extraction technics for total flavonoids from Astragalus.Methods The orthogonal design was conducted to investigate the main cellulose enzymatic extraction of total flavonoids from Astragalus and the best extraction technics was determined.Results The optimum conditions cellulose enzymatic extraction of total flavonoids from Astragalus were as follows： enzymolysis time 120min,hydrolysis temperature 30 ℃,enzyme volume 8mg,PH value 4.5.Conclusion This method is simple and feasible.It is an effective way for extraction of total flavonoids from Astragalis.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第10期2478-2480,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家"十五"科技攻关计划重大项目(No.2004BA721A39)
广州市科技计划项目(No.2005Z3-E5071)
关键词
纤维素酶
黄芪
总黄酮
正交设计法
Cellulase
Astragalus
Total flavonoids
Orthogonal design
