摘要
目的 :探讨黄芪总黄酮 (TFA)是否具有抗突变作用。方法 :设 3个浓度的药物组和突变物对照组 ,采用小鼠骨髓嗜多染红细胞微核实验、小鼠睾丸精子畸变实验和体外哺乳类动物细胞V79/HGPRT基因突变试验。结果 :与突变物对照组比较 ,TFA不影响小鼠体重增长 ;高剂量组的TFA可明显抑制环磷酰胺诱发的小鼠骨髓嗜多染红细胞微核发生和体外培养的哺乳类动物细胞V79/HGPRT基因突变 ,但对丝裂酶素C诱发的小鼠睾丸染色体畸变无统计学抑制作用。结论 :TFA具有一定的抗突变作用。
Objective: To investigate the anti-mutagenic action of the total flavonoids of Astragalus (TFA). Method: Three groups of concentrations of TFA and one inducer group were used. The anti-mutagenic action of TFA was studied by experiments in vitro and in vivo including miconucleolus assay, semen teraogenesis test and gene mutagenesis of V79/HGPRT. Result: TFA did not affect the weight of the mice during the experiment period. The high concentration of TFA could significantly reduce cyclohophamide-induced miconucleolus number and gene mutagenesis of V79/HGPRT compared with inducer group. However, TFA did not make statistic change in the semen teraogenesis induced by mitomycin C. Conclusion: TFA has antimutagenesis effect.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第12期1164-1167,共4页
China Journal of Chinese Materia Medica
基金
国家教委回国人员科研启动基金
关键词
黄芪总黄酮
抗突变作用
实验
中药
小鼠
total flavonoids of Astragalus
mutagenesis
micronuclolus assay
semen teraogenesis test
gene mutagenesis
