摘要
目的通过检测婴幼儿外周血树突状细胞和T细胞亚群的数量变化,探讨宫内感染乙型肝炎病毒导致免疫失败的机制。方法采用流式细胞仪技术和三色荧光染色法,对20例宫内感染免疫失败婴幼儿及29例宫内感染免疫成功婴幼儿进行外周血髓源性树突状细胞(MDC)、浆细胞样树突状细胞(PDC)和T细胞亚群检测。结果免疫成功组外周血MDC的百分比为(0.30±0.12)%,绝对数为(20.28±6.82)×106/L;PDC百分比为(0.28±0.12)%,绝对数为(18.01±5.58)×106/L;CD4+百分比(39.55±8.80)%,CD8+百分比(24.62±7.23)%,CD4+/CD8+为1.66±0.51。免疫失败组外周血MDC的百分比为(0.18±0.08)%,绝对数为(12.94±5.97)×106/L;PDC百分比为(0.16±0.09)%,绝对数为(11.75±7.36)×106/L;CD4+百分比(32.94±7.58)%,CD8+百分比(29.61±7.08)%,CD4+/CD8+为1.13±0.50。免疫失败组婴幼儿外周血2种DC的百分比、绝对数、CD4+百分比、CD8+百分比、CD4+/CD8+与免疫成功组比较差异均有统计学意义(P<0.05)。结论婴幼儿外周血树突状细胞和T细胞亚群数量的改变可能是宫内感染乙型肝炎病毒导致乙肝疫苗免疫接种失败的原因之一。
Objective To study the mechanism of compromised immunity due to hepatitis B virus （HBV） intrauterine infection via the detection of Dendritic cells （DC） and T cells subsets in peripheral blood. Methods The DC subsets and T cell subsets of peripheral blood from 29 patients with immunity and 20 with compromised immunity were analyzed by flow eytometry and three-color fluorescence staining. Results The percentage of MDC, PDC, CD4＋ and CD8＋ cells was （0.30±0.12） %, （0.28±0.12） %, （39.55± 8.80） %, and （24.62± 7.23） % , indicating immunity in the immune group. The absolute number of MDC and PDC was （20.28±6.82）×10^6/L and （18.01±5.58）×10^6L, respectively. The CD4＋/CD8＋ value was 1. 66 ± 0. 51. The percentage of MDC, PDC and CD4＋ cells in the immunocompromised group were significantly lower than those in the immune group （P〈0.05）, as was the CD4＋/CD8＋ value. However, the percentage of CD8＋ cells in the immune group was significantly lower than that in the immunocompromised group （P〈 0.05）. Conclusion Changes in DC subset and T cell subset levels in peripheral blood may be important factors that re- sulted in compromised immunity in infants with HBV intrauterine infection.
出处
《中国病原生物学杂志》
CSCD
2009年第9期641-644,共4页
Journal of Pathogen Biology
基金
山东省计生委资助项目(No.2006-6)
