摘要
目的:探讨干扰素抗病毒应答与慢性乙型肝炎患者外周血树突状细胞(DCs)功能的关系。方法:分别采集23例慢性乙型肝炎患者干扰素治疗前和治疗满4mo时的抗凝外周静脉血,分离外周血单个核细胞(PBMCs),在重组人白细胞介素4和重组人粒细胞-巨噬细胞集落刺激因子的作用下培养7 d使DCs增殖、成熟。以间接免疫荧光流式细胞技术检测DCs的表型;以ELISA法检测DCs单独培养上清液中IL-12的水平;用DCs与HBsAg共同孵育,丝裂霉素C处理后,再与自体PBMCs共同培养,加入H-TDR,收集细胞测定cpm值。实验中以8例正常健康人作为对照。结果:干扰素完全应答组DCs表面CD,HLA-DR和ICAM-1的表达较治疗前均显著增加(均为P<0.05),且CD,CD,HLA-DR和ICAM-1的增高与无应答组相比具有显著性意义(分别为P<0.05、P<0.01、P<0.01和P<0.05);完全应答组DCs分泌IL-12的水平在治疗后显著增加(P<0.01);DCs的抗原提呈作用在治疗前、后,完全应答组比无应答组均显著增强(P<0.01和P<0.001),而部分应答与无应答组之间无显著性差异(P>0.05)。结论:慢性乙肝患者干扰素的抗病毒应答与外周血DCs的功能状态有关,干扰素能显著促进患者DCs功能的改善,进而可能增加患者对治疗的应答。
Objective: To study the relationship between therapy response to interferon and the functional status of peripheral blood dendritic cells (DCs) from patients with chronic hepatitis B. Methods: The peripheral blood samples were obtained from 23 patients at the time of interferon pretreatment and posttreatment for 4 months, respectively. The peripheral blood mononuclear cells (PBMCs) were isolated and cultured, and recombinant human IL-4 and GM-CSF were added to the cuLtures. After cuLtured for 7 days, DCs were harvested for the following studies. CD CD , HLA-DR as weLL as ICAM-1 on the membrane of DCs were detected by indirect immunofiuorescent flow cytometry. DCs were cultured for another 3 days, and the supernatants were collected and the levels of IL-12 were detected with ELISA. DCs were incubated with HBsAg, following treatment with mitomycin C, then DCs were collected and mixed with autogenous PBMCs and cocultured. At last, H TDR was added to the culture, and the cpm value was detected for all the harvested cells. 8 healthy individuals were regarded as controls. Results: in interferon complete responder group, the levels of CD , HLA-DR and ICAM-1 on DCs significantly increased in posttreatment group compared with those in pretreatment one (P<0.05 in all). Likewise, the levels of CD , CD , HLA-DR and ICAM-1 on DCs in complete responder group were significantly higher than those in nonresponder one, respectively (P0.05, P<0.01, P<0.01 and P<0.05). In complete responder group, the level of IL-12 secreted by DCs after treatment markedly increased than that before treatment (P<0.01). Regarding to the capacities of DCs antigen presenting in complete responder group, both in pretreatment group and in posttreatment one, they were significantly stronger than those in nonresponder group, respectively P<0.01 and P<0.001 . There was no significant difference between the partial responder group and the nonresponder one P>0.05 . Conclusions: The resuLts indicate that there may be a close relationship in the response to int
出处
《抗感染药学》
2004年第2期60-63,共4页
Anti-infection Pharmacy
关键词
病毒性肝炎
干扰素
应答
树突状细胞
hepatitis B
chronic
interferon
response
dendritic cell
