摘要
采用鞣酸—柠檬酸钠还原法制备了不同颗粒大小（如φ6nm、φ10nm、φ15nm）、不同种类的胶体金标记抗小鼠IgG、抗人IgG及SPA等探针。所制备的胶体企探针直径达到标记要求，颗粒大小均匀，稳定性好。用该探针检测健康人外周血淋巴细胞表面抗原，如CD3、CD4和CD8等T-淋巴细胞亚类抗原，其本底低，对比度好，阴阳性分明，优于常规的免疫荧光法。
Various particle size and varieties of colloidal gold probes, such as anti-mice IgG, anti-haman IgG and SPA, were prepared by tannic acid-sodium citrate reduction method. Each piobe showed good stability, and even particle size which reached the requirement for label. Health human peripheral blood lymphocyte surface antigens, sach as CD3,CD4 and CD8 were detected by the probes. The result indicated that the method showed low background, good contrast, and distinct threshold between the positivity and negativity, and was superior to routine immunofluorescence.
出处
《中国生物制品学杂志》
CAS
CSCD
1994年第4期153-155,共3页
Chinese Journal of Biologicals
关键词
胶体金
探针
T细胞亚群
免疫金银染色法
Colloidal gold probe T cell subgruop Immuno-gold silver staining
