摘要
目的探讨趋化因子CXCL12与其受体CXCR4在肝细胞癌（HCC）的表达及其与肝内浸润转移的关系。方法采用逆转录-聚合酶链反应和免疫组织化学SP方法分别检测37例肝内转移HCC标本中原发灶、转移灶及其配对癌旁组织mRNA和蛋白表达。结果在37例肝内转移HCC中，CXCL12和CXCR4 mRNA和蛋白表达一致。原发灶、转移灶和癌旁组织均有表达，转移灶CXCR4表达高于原发灶（P〈0．05），原发灶和转移灶CXCL12表达低于癌旁组织（P〈0．01），CXCL12、CXCR4表达与HCC肝内转移有显著相关。结论CXCL12可能诱导CXCR4激活的肝癌细胞的侵袭，二者可能是HCC重要的免疫学分期、预后指标和新的肝内转移治疗途径。
Objective To investigate the expression of ehemokine CXCL12 and its chemokine receptor CXCR4 in hepatoeellular carcinoma （HCC） , and their correlations with intrahepatie metastasis （IM）. Methods We compared the expression levels of CXCLI2 and CXCR4 among the primary cancer,metastases and tumoradjacent tissues in 37 HCC specimens with IM. Their expression levels were detected by reverse transcription polymerase chain reaction （RT- PCR） and immunohistoehemistry. Results There was the expression of CXCL12 and CXCR4 in primary cancer,metastases and tumor - adjacent tissues. The expression level of CXCR4 in metastases was higher than in primary cancer （ P 〈 0.05） , and that of CXCL12 in primary cancer and metastases was significantly lower than in tumor -adjacent tissues （ P 〈 0. 01 ）. Their expression was significantly correlated to IM of HCC. Conclusion CXCL12 may induce the invasion of hepatoma carcinoma cells by activating CXCR4. They may be important predictors and show us some new therapeutic methods for IM and immunologic classification of HCC.
出处
《临床外科杂志》
2009年第8期525-527,共3页
Journal of Clinical Surgery
