摘要
目的:对黔产三尖杉抗肿瘤活性成分进行研究。方法:用硅胶、葡聚糖凝胶(Sephadex LH-20)柱色谱等方法进行分离纯化,通过理化性质及光谱数据确定化合物的结构;采用磺酰罗丹明(SRB)法和四甲基偶氮唑蓝(MTT)法对粗提物和部分单体化合物进行抗肿瘤活性的测定。结果:分离得到11个化合物,它们分别是:芹菜素(Ⅰ)、β-谷甾醇(Ⅱ)、乙酰三尖杉碱(Ⅲ)、柯伊利素(Ⅳ)、桥氧三尖杉碱(Ⅴ)、正三十一烷醇(Ⅵ)、7,3′,4′-三羟基黄酮(Ⅶ)、柳杉酚(Ⅷ)、三尖杉碱(Ⅸ)、台湾三尖杉碱(Ⅹ)和海南粗榧内酯hainanolide(Ⅺ);活性筛选结果表明,部分化合物具有一定的抗肿瘤活性。结论:初步阐明了黔产三尖杉中的主要化学成分,其中化合物Ⅱ、Ⅵ～Ⅷ为首次从该植物中分离得到;化合物(Ⅺ)对人肺癌细胞株(A549)和人白血病细胞株(K562)有较好的抑制活性。
Aim： To study the antitumor activities of the constituents of Cephalotaxus fortunei distributed in Guizhou province. Methods： The constituents were isolated by column chromatography and identified by physical and spectral analysis. Meanwhile, the anti-tumor activities of some compounds were evaluated by sulforhodamine B（SRB） and MTT assay. Results： Eleven compounds were isolated and identified as apigenin （Ⅰ）,β-sitosterol （Ⅱ）, acetylcephalotaxine （Ⅲ）, chrysoeriol （Ⅳ）, drupacine （Ⅴ）, 1-hentriacontanol （Ⅵ）, 7, 3＇, 4＇-trihydroxyflavone （Ⅶ）, sugiol （Ⅷ）, cephalotaxine （Ⅸ）, wllsonine （Ⅹ）, and hainanolide （Ⅺ）, respectively. Biological screening results demonstrated that some of the tested compounds exhibited the antitumor activities in vitro. Conclusion： Compounds Ⅱ, Ⅶ-Ⅷ were isolated from this plant for the first time. Compound XI has a better inhibitory activity on cell line A549 and K562 .
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2009年第3期209-212,共4页
Journal of China Pharmaceutical University
基金
基金项目973计划前期研究专项课题(No.2007CB516813)
贵州省中药现代化科技产业研究开发项目(No.20065041)~~
关键词
三尖杉
化学成分
抗肿瘤活性
Cephalotaxus fortunei
chemical constitutes
antitumor activity
