摘要
以对杂草反枝苋种子萌发及根、茎生长的抑制作用为指标,提取、分离、鉴定了中国粗榧C ephalotaxus sinensisL i.枝叶中的除草活性成分。中国粗榧枝叶乙醇提取物的酸水溶液用苯萃取,萃余相碱化并用氯仿萃取得总生物碱。以硅胶及氧化铝柱层析从中国粗榧总生物碱中分离出了3个生物碱类化合物H1、H2和H3,其结构依次鉴定为桥氧三尖杉碱、三尖杉碱及11-羟基三尖杉碱。在1.00mg/mL的供试浓度下,H1对反枝苋种子根、茎生长的抑制率分别为86.1%和82.4%,H3对反枝苋种子根、茎生长的抑制率分别为84.2%和77.1%,H2除草作用较弱。测定结果表明,萃取所得总生物碱为中国粗榧的主要活性成分。
Herbicidal components of Cephalotaxus sinensis against Amaranthus retroflexus were isolated by bioassay-guided separation. The main active components were alkaloids in chloroform partition derived from the ethanol extract. Drupacine, cephalotaxine and 11-hydroxycephalotaxine were isolated from the chloroform partition first by silica gel column chromatography （CC） and then by aluminum oxide CC respectively, and identified by IR, MS ,1H NMR. The herbicidal activity of drupacine and 11-hydroxycephalotaxine were much higher than cephalotaxine. The inhibitory rates of drupacine and 11 -hydroxycephalotaxine to the growth of root and stem of Amaranthus retroflexus were nearly or more than 80% at 1 mg/mL concentration.
出处
《农药学学报》
CAS
CSCD
2006年第1期91-94,共4页
Chinese Journal of Pesticide Science
基金
西北农林科技大学青年专项基金
西北农林科技大学人才基金
国家"十五"科技攻关项目(2002BA516A04)
