摘要
［目的］建立万应跌打酒中东莨菪素的HPLC分析方法。[方法］采用Diamonsil C18（5 μm×250 mm×4.6 mm）色谱柱，以乙腈-0.1％磷酸水溶液为流动相梯度洗脱，流速为1.0 ml/min，检测波长为360nm，柱温为35 ℃。［结果］ 东莨菪素含量9.008-90.080（甲醇）μg/ml 范围内与其峰面积呈良好的线性关系，r＝0.999 9；平均回收率为98.83％（RSD＝1.18％）。10批万应跌打酒中东莨菪素的含量在0.03-0.12 mg/ml。［结论］该方法科学准确、操作简单、重复性好，可用于万应跌打酒的质量控制。
[ Objective] The research aimed to develop a quantitative method for the determination of scopoletin in Wanying Dieda Wine. [ Method] The samples were separated at 35 ℃ on a Diamonsil C18 column eluted with acetonitrile-phosphoric acid as the mobile phase （ gradient elution）. Flow rate was set at 1. 0 ml/min and the detection wavelength was set at 360 nm. [ Result] The scopoletin content showed a linear relationship with the peak area within the methanol range from 9. 008 to 90. 080 μg/ml （ r = 0. 999 9） and the average recovery was 98.83% , with RSD of 1.18%. 10 batches of the samples were determined and the contents of scopoletin in Wanying Dieda Wine were fluctuated from 0.03 to 0.12 mg/ml. [ Conclusion] The method is accurate, simple, repeatable and it can be used for tire quality control of Wanying Dieda Wine.
出处
《安徽农业科学》
CAS
北大核心
2009年第29期13998-14000,共3页
Journal of Anhui Agricultural Sciences
关键词
万应跌打酒
丁公藤
东莨菪素
HPLC
含量测定
Wanying Dieda Wine
Erycibe obtusifolia Benth.
Scopoletin
HPLC
Content determination
