摘要
[目的]建立同时测定平菇及培养料中多菌灵和甲基硫菌灵残留的快速分析方法。[方法]平菇及培养料样品经乙酸乙酯提取、C18色谱柱分离后,采用带有紫外检测器的HPLC法检测多菌灵和甲基硫菌灵的残留量,然后在样品中加入一定浓度的多菌灵和甲基硫菌灵标准工作溶液进行添加回收试验。[结果]通过HPLC紫外检测,在15min内实现了2种农药的同时分离测定。2种农药的标准工作溶液的进样量与色谱峰面积存在显著线性关系。平菇中多菌灵的添加回收率和相对标准偏差为87.83%～95.72%和2.21%～16.34%,甲基硫菌灵的相应指标为88.65%～92.49%和4.33%～16.32%;在平菇的培养料中多菌灵的相应指标为84.07%～90.61%和8.46%～12.92%,甲基硫菌灵的相应指标为78.83%～88.65%和7.90%～9.76%。[结论]该分析方法的准确性和灵敏度均达到农药残留分析的要求。
[ Objective ] The study aimed to establish the rapid analysis method for determining earbendazol and thiophanate-methyl residue in Pleuotus ostreatus and its culture material simultaneously. [ Method ] After the samples of P. ostreatus and its culture material was extracted by ethyl acetate and was separated by C18 column, the earbendazal and thiophanate-methyl residue in 2 samples were determined by HPLC with UV detector. Afterward, the standard working solutions of earbendazol and thiophanate-methyl were added into 2 samples to make the additive recovery experiments. [ Result] These 2 pesticides were separated and detected within 15 min simultaneously through determination by HPLC with UV detector. The injection volume of standard working solutions of 2 pesticides had significant linear relationship to chromatographic peak area. In P. ostreatus, the recoveries and relative standard deviation of earbendazol were 87.83% - 95.72% and 2.21% - 16.34% resp. and those of thiophanate-methyl were 88.65% - 92.49% and 4.33% - 16.32% resp. In culture material, the recoveries and relative standard deviation of earbendazol were 84.07% -90.61% and 8.46% - 12.92% resp. and those of thiophanate-methyl were 78.83% - 88.65% and 7.90% -9.76% resp. [ Conclusion ] The accuracy and sensitivity of this analytical method reached the request of pesticide residue analysis.
出处
《安徽农业科学》
CAS
北大核心
2009年第30期14573-14575,14580,共4页
Journal of Anhui Agricultural Sciences
关键词
高效液相色谱
平菇
培养料
多菌灵
甲基硫菌灵
HPLC
Pleuotus ostreatus
Culture material
Carbendazol
Thiophanate-methyl
