摘要
采用"龙芋一号"茎尖为外植体进行脱毒培养,探索6-BA、NAA、蔗糖的质量浓度及固体培养、液体培养对"龙芋一号"茎尖培养的影响。实验结果表明,外植体表面灭菌采用体积分数为50 ml/L的次氯酸钠溶液消毒10 min的效果较好;适宜的茎尖分化培养基、增殖培养基、生根培养基分别为MS+6-BA1.0 mg/L+NAA0.5 mg/L、MS+NAA0.2 mg/L+6-BA0.5 mg/L、MS+6-BA0.1 mg/L+NAA0.5 mg/L+蔗糖20 g/L;生根培养采用液体培养基,可以降低生产成本;生根培养基中蔗糖的质量浓度为50 g/L有利微型芋的形成。
In vitro shoot tip tissue virus-free culture was studied, using the shoot-tips from the colocasia esculenta variety of Longyu No. 1 as the first explants. Our investigation involved evaluating the effect of concentration of 6-BA, NAA, sucrose and solid, liquid culture on shoot tip. The antiseptic results showed that the optimum antiseptic concentration was 50ml/L NaClO and the time was 10 min. The appropriate medium for buds differentiation was MS＋6-BA1.0mg/L ＋ NAA0.Smg/L. The best medium of proliferation was MS＋NAA 0.2mg/L＋6-BA 0.Smg/L. The best medium for rooting was MS＋6BA0.1mg/L ＋NAA0.5mg/L ＋20g/L sucrose. Without the expensive agar, using liquid culture canreduce the cost and gain better growth than solid culture. It was good for the growth of micro-taro if applying 50g/L sucrose.
出处
《闽西职业技术学院学报》
2009年第3期103-106,共4页
Journal of Minxi Vocational and Technical College
关键词
“龙芋一号”
茎尖培养
快繁技术
Longyu No. 1
stem tip culture
rapid propagation technology
