摘要
目的观察姜黄素和地塞米松对百草枯致肺纤维化大鼠胶原沉积、转化生长因子一p1（TGF一口1）表达的影响。方法将80只雄性Wistar大鼠随机分为4组，每组20只。百草枯组用百草枯50mg／kg一次性灌胃染毒，1h后腹腔注射生理盐水1ml；姜黄素组染毒1h后腹腔注射姜黄素200mg／kg；地塞米松组染毒1h后腹腔注射地塞米松3mg／kg；对照组用1ml生理盐水灌胃，1h后再腹腔注射生理盐水1ml。各组每日用药1次，直至处死动物前1d为止。各组于1、3、7、14d随机处死5只动物，取肺组织进行苏木素-伊红（HE）染色、Masson染色，观察病理学改变；采用免疫组化染色，检测肺组织中TGF-β1表达水平。结果地塞米松组和姜黄素组大鼠肺组织胶原沉积较百草枯组明显减少。百草枯组、地塞米松组和姜黄素组7d和14d时TGF-β1表达均显著高于对照组，且地塞米松组和姜黄素组明显低于百草枯组（P〈0．05或P〈0．01）；而地塞米松组和姜黄素组各时间点TGF-β1表达则无明显差异（P均〉0．05）。结论姜黄素和地塞米松均可减轻百草枯所致大鼠肺泡炎和肺纤维化，其作用机制可能与抑制TGF-β1有关。姜黄素和地塞米松治疗效果无明显差异，但姜黄素比地塞米松副作用少，更具有临床应用价值。
Objective To observe the effect of curcumin （姜黄素） and dexamethasone on the expression of transforming growth factor-β1（TGF-β1） and amount of collagen deposition in the pulmonary fibrosis caused by paraquat in rats. Methods Eighty male Wistar rats were randomly divided into four groups （each n= 20）. At the beginning of the experiment, each rat in paraquat, curcumin and dexamethasone groups was treated with paraquat （50 mg/kg）, ancl in normal control and paraquat groups, treated with 1 ml saline intra-gastrieally. An hour after the poisoning, eurcumin group received curcumin （200 mg/kg） and dexamethasone group received dexamethasone （3 mg/kg） intra-peritoneally, and 1 ml saline was given to the normal control and paraquat groups via the same route. The rats in each of the above groups were treated with their respective drugs or saline once a clay until one clay before death. On each of the following days, the 1st, 3rd, 7th and 14th day, 5 rats from each group were randomly killed; the lung was incised to make pathological sections which were stained with hematoxylin-eosin （HE）, Masson and by immunohistochemical technique to show the expression of TGF-β1. Results The collagen deposition in eureumin and dexamethasone treatment groups was obviously lighter than that in paraquat group. The lung tissue expression of TGF--β1 on the 7th and 14th day was significantly increased in paraquat, eurcumin and dexamethasone groups, hut in eurcumin and dexamethasone treated groups, it was significantly lower than that in the paraquat group （P〈0.05 or P〈 0. 01）. There was no obvious difference between the two drug treatment groups in the expression of TGF-β1 at each time point （all P〈0. 05）. Conclusion Curcumin and dexamethasone both can alleviate pulmonary alveolitis and fibrosis induced by paraquat in rats, the mechanism is possibly related to the inhibition of TGF-β1. Although the therapeutic effect of curcumin and dexamethasone has no significant difference, curcumin has le
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第5期299-301,I0002,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
