摘要
目的探讨甲基泼尼松龙对急性肺损伤(ALI)大鼠型前胶原(PC)的影响。方法将45只大鼠按随机数字表法分为内毒素损伤组(损伤组)、甲基泼尼松龙干预组(干预组)和对照组,每组15只。通过静脉注射内毒素(6mg/kg)制备大鼠ALI模型,于制模后1、3和14d各处死5只,观察各组支气管肺泡灌洗液(BALF)中细胞总数、中性粒细胞(PMN)分类、巨噬细胞分类及血清和BALF中PC水平变化。结果损伤组1d BALF细胞总数、PMN分类及血清和BALF中PC水平即明显升高,与对照组比较差异均有显著性(P均<0.01),干预组上述指标均明显低于损伤组,差异有显著性(P<0.05或P<0.01)。结论甲基泼尼松龙抑制内毒素所致ALI大鼠血清和BALF中PC水平升高,对ALI早期肺纤维化有抑制作用。
Objective To observe the effect of methylprednisolone on procollagen type Ⅲ of rats with acute lung injury （ALI） induced by endotoxin. Methods Forty -five rats were randomly divided into three groups： endotoxin group, endotoxin plus methylprednisolone group, and control group, with 15 rats in each group. The model of ALI in rat was reproduced by intravenous injection of endotoxin in the dose of 6 mg/kg. On 1, 3, 14 days after the onset of ALI in rats, total cell number, classification of polymorphonuclear neutrophill （PMN）, macrophages and procollagen type Ⅲ in serum and bronchoalveolar lavage fluid （BALF） were determined by radioimmunology. Results Total cell number, classification of PMN and macrophages in BALF as well as procollagen type Ⅲ level in serum and BALF were obviously increased in endotoxin group on the first day, then decreased gradually （all P〈0.01）. Methylprednisolone inhibited the increase of the above-mentioned parameters （P〈0.05 or P〈0.01）. Conclusion Methylprednisolone can inhibit the increase of the procollagen type Ⅲ levels in serum and BALF in rats with acute lung injury induced by endotoxin.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第8期459-461,共3页
Chinese Critical Care Medicine
基金
国家"十五"科技攻关计划项目(2003BA712A0701)
