摘要
目的利用动物模型观察实验型阻塞型睡眠呼吸暂停综合征小型猪的氧化应激反应,以探讨睡眠呼吸暂停致动脉粥样硬化的可能机制。方法中国小型猪16头,分为阻塞型睡眠呼吸暂停(OSA)组和对照组,每组8头。OSA组用凝胶注射法制作动物模型。分别于制作模型前及12周后取实验动物静脉血检测黄嘌呤氧化酶(XOD)、超氧化物岐化酶(SOD)及丙二醛(MDA)。结果(1)实验后,OSA组XOD水平明显升高,前后分别为(3.71±0.07)U/L和(4.82±0.08)U/L,两者相比差异有统计学意义,对照组XOD水平无明显变化。实验后OSA组与对照组XOD值相比差异有统计学意义。(2)实验后,OSA组的SOD水平明显降低,前后分别为(142.33±6.82)NU/ml和(121.21±1.93)NU/ml,两者相比差异有统计学意义;MDA水平明显升高,前后分别为(3.66±0.47)μmol/L和(5.59±0.75)μmol/L,两者相比差异有统计学意义。对照组SOD水平及MDA水平无明显变化,实验后OSA组与对照组二指标相比差异有统计学意义(P<0.05)。结论实验性阻塞型睡眠呼吸暂停使动物机体处于氧化应激状态,产生了大量的氧自由基,增多的氧自由基介导了一系列病理生理反应,促进了动脉粥样硬化的发生和发展。
Objective To explore the possible mechanism of sleep apnea causing atherosclerosis by observe the oxidative stress reaction in the animal models with experimental obstructive sleep apnea syndrome. Methods Sixteen Chinese miniature pigs were equally divided into obstructive sleep apnea （OSA） group and control group. Animal models were made by gel injection method in OSA group. Before surgery and 12 weeks after surgery, venous blood was obtained from experimental animals to measure xanthine oxidase （XOD）, superoxide dismutase （SOD） and malondialdehyde （MDA）. Results （1） After surgery, the level of XOD was obviously elevated in OSA group, increasing from（3.71±0.07） to （4.82±0.08） U/L. Significant difference existed in the XOD level between before and after surgery. No significant change in the level of XOD was found in control group after surgery. There was significant difference in the XOD level between OSA and control groups after surgery. （2） After surgery, the level of SOD depressed from （142.33± 6.82） to （121.21± 1.93） NU/ml in OSA group. Significant difference existed in the SOD level between before and after surgery . The level of MDA was obviously elevated in OSA group after surgery, increasing from （3.66±0.47） to （5.59±0.75） /μmol/L. Significant difference existed in the MDA level between before and after surgery. In control group, there were no significant changes in the levels of SOD and MDA after surgery, Significant differences existed in SOD and MDA between OSA and control groups after surgery（P（0. 05）. Conclusion Expermental obstructive sleep apnea can put the animals into oxidative stress state and produce a large amount of oxygen free radicals. Increased oxygen free radicals induce a series of pathophysiological reactions and promote the initiation and development of atherosclerosis.
出处
《中华老年多器官疾病杂志》
2009年第4期355-358,363,共5页
Chinese Journal of Multiple Organ Diseases in the Elderly
基金
国家自然科学基金项目(30873412)
军队"十五"重点资助项目(02Z009)
