摘要
为探讨环鸟苷酸(cGMP)在噻环乙胺全麻分子学机理中可能的作用,48只SD大鼠,随机均分为对照组、麻醉组、恢复Ⅰ组和恢复Ⅱ组,用放射免疫法分别测定各脑区的cGMP含量。结果显示,大鼠腹腔注射噻环乙胺30mg/kg后,麻醉组大脑皮层、海马、丘脑的cGMP含量明显降低,分别较对照组降低了35.30%(P<0.01),26.48%(P<0.01),40.67%(P<0.01),而在恢复Ⅰ组和恢复Ⅱ组cGMP含量明显恢复(与对照组相比,P>0.05)。在噻环乙胺麻醉全过程中脑干、小脑的cGMP含量未发生明显的变化。这表明,cGMP参与了噻环乙胺全麻作用产生的分子学机理的调控,噻环乙胺全麻作用可能与抑制大脑皮层、海马和丘脑等脑区cGMP释放有关。
To dynamicly observe the effect of tiletamine on cGMP content in different brain rigions of rats,and investigate the role of tiletamine in the molecular mechanisms of general anaesthesia mediated by tiletamine, 48 SD rats were randomly divided into control group, maintenance of anesthesia group, recovery from anesthesia Ⅰ group and recovery from anesthesia Ⅱ group. The cGMP content in different brain rigions was measured by radiommunoassay （RIA）. The rats were administered intraperitoneally （ip） tiletamine 30 mg/kg,and then cGMP content of maintenance of anesthesia group in cerebral cortex,the hippocampus and the thalamus was obviously decreased,and compared with control group,the results decreased by 35.30% （P〈0.01）,26.48%（P〈0.01） and 40.67%（P〈0.01）, respecially. But the cGMP content of recovery from anesthesia Ⅰ group and recovery from anesthesia Ⅱgroup was significant recovery（compared with control group,P〉0.05）. The cGMP content in the brain stem and the cerebellum was not obviously changed in the process of general anesthesia of tiletamine, cGMP participate in the molecular mechanisms of general anaesthesia mediated by tiletamine. The anesthesia effects of tiletamine may relate to inhibited cGMP＇ release of cerebral cortex, the thalamus, and the hippocampus.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第9期1201-1203,1207,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30671552)
黑龙江省自然科学基金重点资助项目(ZJN0503-01)
