摘要
目的:建立栀子及其炮制品中熊果酸的测定放法,考察栀子炮制前后熊果酸的含量变化。方法:采用高效液相色谱法,色谱柱为Agilent C18(4.6mm×150mm,5μm),流动相为流动相:甲醇-0.1%磷酸(86:14);检测波长220 nm。结果:熊果酸在0.120～1.200μg之间线性关系良好,平均加样回收率97.54%,RSD为1.8%。结论:方法用于栀子及其炮制品中熊果酸含量测定简单方便,重复性好。
Objective： To analysis the content change of ursolic acid in three kind of Gardenia jasminoids Ellis slices processed. Method：A HPLC method was developed, the chromatographic conditions are as fellow： Agilent C18 column, 4.6mm × 150mm, 5 μ m, methanol-1.0% phosphoric acid （86：14） as mobile phase, detection wavelength at 220nm, the flow was 1.0mL/min. Result：The content of ursolic acid changed markedly in three sorts slices. Conclusion：The average content of ursolic acid in Sheng slice is 0.053%, 0.048% in Chaohuang slice and 0.027% in Chaojiao slice. The content of ursolic acid reduce sharply after processed. This method is scientific to control the processing degree of Gardenia jasminoids Ellis slices withthe content of ursolic acid.
出处
《辽宁中医药大学学报》
CAS
2009年第9期166-167,共2页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
HPLC法
栀子
炒栀子
焦栀子
熊果酸
HPLC
Gardenia jasminoids Ellis
Processed slices
Ursolic acid
