摘要
目的建立粗叶悬钩子根部总生物碱的含量测定方法。方法采用酸性染料比色法,测定粗叶悬钩子根部总生物碱的含量。结果浓度在6.018～108.3μg时呈现良好的线性关系,其回归方程A=1548.24X-1.0254,r=0.9995,测得粗叶悬钩子根部总生物碱含量为0.81%,平均回收率为97.82%,RSD为0.82%。结论本方法简便,可用于粗叶悬钩子总生物碱的含量测定。
Objective To establish a method for assaying of total alkaloids from the root of Rubus alceaefolius Poir. Methods The total alkaloids content was determined by acid dye colorimetry. Results The calibration curve was linear in the range of 6.018-108.3 μg for alkaloids, and the regression equation was A=1548.24X-1.0254, r=0.9995. The total alkaloid content was 0.81%, the average recovery was 97.82%, RSD=0.82% （n=5）. Conclusion The method is simple and can be used to determine the content of total alkaloids from the root of Rubus alceaefolius Poir
出处
《福建中医学院学报》
2008年第5期28-30,共3页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省自然科学基金项目(2006J0109)
福建省教育厅重点课题(JA07111)
关键词
粗叶悬钩子
生物碱
酸性染料比色法
含量测定
Rubus alceaefolius Poir
alkaloids
acid dye colorimetry
determination
