摘要
目的:研究妊娠期和哺乳期染镉(Cd)对雄性子代性腺发育的影响及作用机制。方法:SD孕鼠16只随机分为4组:2个染Cd组(A组、B组)、正常对照组(C组)和阳性对照组(D组),每组4只。A组和B组于妊娠第9日、第11日、第13日及产后隔日腹腔注射氯化镉(CdCl2),剂量分别为每次1.2mg/kg、1.5mg/kg;C组腹腔注射等量生理盐水;D组于妊娠9￣17d及产后隔日皮下注射己烯雌酚(DES)每次100μg/kg。出生后30d,光学显微镜、电子显微镜观察仔鼠睾丸形态及组织学改变,免疫组织化学法检测睾丸细胞色素P450胆固醇侧链裂解酶(P450scc)和雌激素受体α(ERα)的表达水平,放射免疫法测定血清睾酮、FSH、LH含量。结果:与C组比,B组睾丸质量和体积减小(P<0.05)。A组、B组和D组睾丸生精上皮均发生不同程度的发育阻滞和超微结构损伤,血清T和LH含量均较C组显著下调(P<0.001),间质细胞中P450scc表达减弱(P<0.05)。B组、D组的精原细胞和间质细胞中ERα表达较C组有所增强(P<0.05)。结论:Cd能对胚胎期至青春期前雄性大鼠性腺发育过程产生干扰。
Objective：To investigate the influence of cadmium exposure during gestation and lactation on the gonadal development of off-spring male rats.Methods：Sixteen pregnant SD rats were randomly divided into two CdCl2 treated groups（group A and group B）,normal control group（group C）,and positive control group（group D）,4 rats in each group.On the day 9,11,13 during the pregnant period and every other day during lactation respectively,group A or B was intraperitoneally injected with CdCl2（1.2 mg/kg or 1.5 mg/kg）,group D was subcutaneously injected with DES（100 μg/kg）on the day 9-17 during the pregnant period and every other day during lactation respectively,group C was given equivalent normal saline.On the day 30 after parturition,the testes of off-spring male rats were obtained for correlative morphological measure,immunohistochemistry of P450scc and ERα,light microscope and electron microscope observation.Meanwhile,serum sex hormones were determined by radioimmunity.Results：The weight and volume of testes were both decreased in group B compared with group C,（P0.05）.The developmental arrest and ultrastructural damage of spermatogenic epithelium were observed in groups A,B and D.Meanwhile,serum T and LH were significantly down-regulated corresponding in groups A,B and D compared with group C（P0.001）,and the positive products of P450scc were significantly decreased,accordingly（P0.05）.Whereas the positive products of ERα were increased in spermatogonia and Leydig cells in group B and group D（P0.05）.Conclusion：Cadmium could cause the morphological damage and functional disorder on male gonadal development during embryonic period to impuberal.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2009年第8期494-501,共8页
Reproduction and Contraception
关键词
镉(CD)
性腺
发育
雄性
cadmium（Cd）
sexual gland
development
male
rat
