摘要
【目的】揭示多氯联苯PCB-118和多溴联苯醚PBDE-209对非洲爪蟾生长发育以及性腺发育的影响。【方法】选择非洲爪蟾第46/47阶段的健康蝌蚪各80只,分别置于0.5μg/L PCB-118、0.5μg/L PBDE-209、0.5μg/LPCB-118和0.5μg/L PBDE-209的混合物中暴露直到变态1个月后,再在不加暴露物的情况下饲养5个月后解剖,取性腺进行形态学和组织学研究。暴露期间,每天记录蝌蚪的生长发育情况和生存率。【结果】PCB-118可使非洲爪蟾的变态时间延长,使雌性的性腺发育延迟。PBDE-209暴露对非洲爪蟾的变态发育、生存率、性腺形态以及性别比无显著影响,但可使睾丸组织中出现早期卵并导致精子排列混乱。PCB-118和PBDE-209联合暴露对非洲爪蟾的变态发育、生存率、性腺形态、性别比以及性腺组织学均未产生明显影响。【结论】PBDE-209对雄性非洲爪蟾的性腺组织学结构有一定影响。
[Objective] The effects of polychlorinated biphenyls and polybrominated diphenyl ethers on the growth and gonadal development of African clawed frogs （Xenopus laevis） were studied. [Method] Xenopus laevis was exposed to 0.5μg/L PCB-118,0.5 μg/L PBDE-209,0.5 μg/L PCB-118 and 0.5 μg/L PBDE-209 mixture in water from NF stage 46/47 to one month postmetamorphosis. Followed by another five months without exposure, the gonads of frogs were examined in gross morphology and histology. During the exposure days,the growth and survival rate were recorded every day. [Result] PCB-118 delayed the metamorphic time and the ovary development. PBDE-209 had no effect on the metamorphosis,survival rate,gross gonadal morphology and the sex ratio,but the histological examination suggested that the tests contained early oocytes and disordered sperms. There were no effects observed in the PCB-118 and PBDE-209 co-exposure group. [Conclusion] The results indicated that PBDE-209 can affect the male gonadal his tologic structure.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第4期31-36,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(2067707420437020)
