摘要
采用Ｗｉｓｔａｒ大鼠６０只，分别饮用含氯化钠１５０ｍｇ／Ｌ（折算每日剂量为１５ｍｇ／ｋｇ）及氟化钠１５０ｍｇ／Ｌ加不同剂量亚硒酸钠的水，二个月后断头处死，取脾脏作光镜、组织化学观察。结果：氟化钠组脾小结与周围界线不清，红髓充血，淋巴细胞核固缩，免疫细胞反应程度降低，糖原ＰＡＳ反应，ＤＮＡ（Ｆｅｕｌｇｅｎ）反应减弱。脾门肥大细胞少。氟化钠加亚硒酸钠２０ｍｇ／Ｌ组（亚硒酸钠折算每日剂量为０２ｍｇ／ｋｇ）脾小结结构清楚，有的可见生发中心，动脉鞘、红髓结构正常。淋巴细胞膜、核结构完整。免疫细胞分布成群，糖原ＰＡＳ反应、ＤＮＡ（Ｆｅｕｌｇｅｎ）反应呈强阳性。脾门肥大细胞多。
Treated with NaF 150mg/L(15mg/kg·d)and different concentration of selenium,60 rats were decopitated after 8 weeksRats spleens were studied by both light microscope and histochemical examinationThe results showed:In group NaF,edge between the splenic corpuscles and other tissues were not clear;the red pulps were filled with bloods;the lymphocytes became pyknosis;the immune cells,the glycogen PAS,DNA(Feulgen)reaction were weakened;the mast cells of lien door became fewerOn the other hand,in group Se 20mg/L(02mg/kg·d),the splenic corpusles were obvious and sometimes germinal centers were seen;the structures of periarterial lymphatis sheath,red pulp,lymphocyte membrane and nucleus were normal;the immune cells were divided in groups;the glycogen PAS,DNA(Feulgen)increased obviously;there were many mast cells in spleen doorThe results demonstrate that Se 20mg/L can protect spleen injured by fluoride 150 mg/L
出处
《职业医学》
1998年第4期4-6,共3页
China Occupational Medicine
