摘要
利用乳酸菌液态发酵得到一种高活性的大豆异黄酮水解酶——乳酸菌β-葡萄糖苷酶,通过单因素试验及正交试验优化了乳酸菌β-葡萄糖苷酶水解大豆异黄酮的条件。正交试验结果显示,当加酶量为25μg/mL,水解温度50℃,水解时间2.0 h,pH6.0时,黄豆苷水解率可达96.51%;在加酶量为25μg/mL,水解温度40℃,水解时间2.0 h,pH5.5时,染料木苷水解率为92.36%。
A high active hydrolysis enzyme of β - glucosidases on soybean isoilavones was obtained by liquid fermentation with lactic acid bacterium. The hydrolysis conditions of soybean isoflavones by β - glucosidases were optimized by single factor and orthogonal tests. The optimal hydrolysis conditions of daidzin were as follows ： concentration of enzyme 25 μg/mL, temperature 50℃, time 2.0 h, pH 6.0. The optimal hydrolysis conditions of genistin were as follows ： concentration of enzyme 25 μg/mL, temperature 40 ℃, time 2.0 h, pH 5.5. Under the optimal conditions, the hydrolysis rate of daidzin and genistin reached 96.51% and 92.36% ,respectively.
出处
《中国油脂》
CAS
CSCD
北大核心
2009年第9期60-63,共4页
China Oils and Fats
基金
黑龙江省"十一五"重大科技攻关项目(GA06B402-4)
大豆生物学教育部重点实验室主任基金项目(SB06A02)
关键词
乳酸菌
Β-葡萄糖苷酶
大豆异黄酮
水解
lactic acid bacterium
β - glucosidases
soybean isoflavones
hydrolysis
