摘要
用发酵黑曲霉得到的β-葡萄糖苷酶水解40%的大豆异黄酮粉,通过正交实验确定最佳水解条件为:加酶量100u,底物浓度20mg/mL,50℃,水解1h。将水解液降温至4℃,4000r/min离心分离30min,离心后沉淀物在-18℃下预冻,于-40℃冷冻干燥,得到固态大豆异黄酮苷元,经检测苷元转化率90.12%、大豆皂苷3.87%、大豆异黄酮94.36%、大豆苷元43.45%、染料木素46.26%。放大实验结果:苷元转化率78.56%、大豆皂苷4.93%、大豆异黄酮88.97%、大豆苷元36.47%、染料木素38.81%。
40% soybean isoflavone powder was obtained by β-glucosidase from Aspergillus niger fermentation. Through orthogonal experiment, the best hydrolyze conditions were as foliows： enzyme 100u,substrate 20mg/mL, 50%, 1 h. The fluid of hydrolyze was centrifuged in 30min, 4%, 4000r/min, the deposition was pre-freezed in -18℃ then -40% freeze-dry. The aglycon transform rate, soybean soap, soybean isoflavones, daidzein, genistein were 90.12%, 3.87 %, 94.36%, 43.45%, 46.26%. After the scale - up, the results were 78.56%, 4.93%, 88.97%, 36.47%, 38.81% respectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第11期152-153,156,共3页
Science and Technology of Food Industry
基金
哈尔滨市科技攻关计划项目(2005AA6CN081)
关键词
大豆异黄酮
水解
苷元
放大
soybean isoflavone
hydrolyze
aglycon
scale-up
