摘要
经农杆菌介导转化葡萄的胚性愈伤组织，研究了影响转化效率的几个因素．结果表明，愈伤组织经１４天预培养，菌液经６～８倍稀释、共培养时保持ｐＨ６．０以及在农杆菌对数期之前８小时加入乙酰丁香酮都显著提高了转化效率．
By using the embryogenic callus of grapevine and Agrobacterium tumefaciense mediated procedures. Some influence factors which affect the transformation of grapevine are observed. Pre_culturing embryogenic callus for 14 days, diluting bacterium concentration 6-8 times, using pH 6.0 co_culture medium and 60μm acetosyringone 8 hours before log phase of bacterium significantly increase the transformation efficiency.
出处
《曲阜师范大学学报（自然科学版）》
CAS
1998年第3期62-64,共3页
Journal of Qufu Normal University(Natural Science)
关键词
葡萄
基因
转化效率
grapevine gene transformation efficiency
