摘要
本研究应用农杆菌介导的方法,采用L9（34）正交试验设计,对影响宁阳大枣基因转化的各项因子进行了分析比较,并着重探索了Ca2＋浓度对侵染转化的影响.结果表明：侵染效率最高的组合为1/2 侵染液浓度,20 min 侵染时间和0 mg/L Ca2＋浓度.Ca2＋浓度在提高宁阳大枣基因转化效率方面没有明显促进效果.Ca2＋浓度仅对平均分化芽数影响较大,浓度为0 mg/L 时,平均分化芽数最多.本试验共获得27 株转化苗,试验转化效率为33.33%.
In this study, the concentration effect of Ca2＋ on transformation of RIP gene （Ribosome Inactivating Protein Gene）cloned from Cinnamomum camphora to Ningyang Jujube was investigated in detail with L9（34） orthogonal design, throughthe analysis of factors which affected the transformation. The results showed that the concentration of Ca2＋ had no crucialeffect on transformation and the best combination was the bacterium solution with 0.4-0.6 OD value was doublediluted,20-minutes infection and without Ca2＋. 27 transformation seedlings were obtained and the transformation rate was33.33%.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2014年第4期481-488,494,共9页
Journal of Shandong Agricultural University：Natural Science Edition
基金
抗枣疯病植原体（MLO）基因转化项目
关键词
农杆菌介导法
香樟核糖体失活蛋白基因
枣疯病
Ca2+浓度
Agrobacterium-mediated transformation
Cinnamomum camphora L. Ribosome-inactivating protein （RIP）
Jujube Witches Broom
Ca2＋ concentration
