摘要
目的:探讨我国石斛属野生种质资源的遗传多样性和亲缘关系。方法:运用RAPD技术对11种野生石斛进行基因组DNA多态性分析,NTSYS软件分析扩增结果。结果:从100条随机引物中筛选出15个随机引物用于供试材料DNA进行随机扩增,共产生152条带,其中多态性片段有129条,多态性百分率占84.9%。采用UPGMA类平均法对扩增结果进行遗传聚类分析,得出反映种间亲缘关系的树状图:在遗传距离=0.6处,可将供试材料分为4类。结论:RAPD技术较好地揭示了石斛属野生种质资源的遗传背景和亲缘关系。
Objective：To investigate the genetic diversity and genetic relationship of wild Dendrobium Germplasm. Methods ：The genetic diversity among 11 wild species of Dendrobium Germplasm was studied by using the random amplification polymorphism DNA. The NTSYS software was used to analyze the markers. Results：15 RAPD primers from 100 random primers were applied to do random amplification. A total of 152 reproducible bands were detected, 129 among which were polymorphic, and the percentage of polymorphic bands was 84. 9%. These results of cluster analysis by used UPGMA method showed that 11 genotypes could be classified into 4 types in genetic distance 0. 6. Conclusion：The result of this research showed that RAPD molecular marker is efficient to study the wild Dendrobium Sw. genetic diversity.
出处
《中华中医药学刊》
CAS
2009年第8期1700-1702,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省科技厅重大科技项目(2006C13014)
