摘要
采用离子交换纤维素色谱DE52柱对微波降解所得λ-卡拉胶(PC2)进行分级,得到3个λ-卡拉胶降解组分(CF1、CF2、CF3)。同时,通过光度法研究了CF1、CF2和CF3清除羟基自由基(.OH)、超氧阴离子(O2-.)和H2O2的活性及体外免疫活性。结果表明,3个多糖组分均具有不同程度的清除自由基及促进淋巴细胞增殖的作用,其中,硫酸基含量高的CF3具有较好的抗氧化活性(CF1、CF2、CF3清除.OH、O2-.和H2O2的IC50值分别为:0.217、0.619、0.087 g/L,0.166、0.214、0.090 g/L和0.602、1.060、0.261 g/L),而分子质量较小的CF1具有较好的体外免疫活性,说明λ-卡拉胶的生物活性不仅与硫酸基含量有关,还与分子质量大小有关。
Three kinds of polysaccharides（CF1, CF2 and CF3） were prepared by anion-exchange column chromatography from degraded lambda-carrageenan PC2 which was obtained by microwave technique. The effects of CF1, CF2 and CF3 on clearing hydroxyl radical, superoxide anion radical and H2O2 have been studied on the paper. The results showed that all three principles had scavenging effects on radical and could advance the immunity of healthy mice. The intensities of the scavenging effects were relative to the molecular weight and sulfute contents. The lower the molecular weight of lambda-carrageenan was, the stronger the immumodulatory was. The higher the sulfute contents of lambda-carrageenan was, the stronger the intensity of scavenging was. The IC50 values of CF1, CF2 and CF3 on clearing hydroxyl radical, superoxide anion radical and H202 were 0. 217, 0. 619, 0. 087 g/L; 0. 166, 0. 214, 0. 090 g/L; 0. 602, 1. 060, 0. 261 g/L respectively.
出处
《海洋科学》
CAS
CSCD
北大核心
2009年第8期58-62,共5页
Marine Sciences
基金
山东省中青年科学家科研奖励基金资助项目(2008BS06012)
烟台市科学技术发展计划项目(2008GGA0020080410084458)
关键词
Λ-卡拉胶
降解
抗氧化活性
分离纯化
体外免疫活性
lambda carrageenan
degraded
antioxidant activity
separation and purification
immumodulatory
