摘要
目的:研究微波法降解得到的不同分子量角叉菜λ-卡拉胶的免疫活性。方法:以离子交换纤维素色谱DE52柱对微波降解所得λ-卡拉胶PC2(product of carrageenan2)进行分级;从实验动物体内获取免疫细胞,采取体外与多糖共同培养的方式,通过淋巴细胞、巨噬细胞增殖实验,中性红吞噬实验以及NO释放实验评价其免疫活性。结果:纯化得到三个硫酸基含量和分子量依次增大的均一多糖分级组分CF1(carrageenan fraction1)、CF2(carrageenan fraction2)、CF3(carrageenan fraction3)。实验结果表明,CF1、CF2、CF3组分均能显著的促进巨噬细胞和ConA诱导的脾细胞增殖,并能增强巨噬细胞吞噬能力和NO的释放。结论:在体外培养研究条件下,λ-卡拉胶降解组分对正常小鼠的免疫能力有促进作用。
The microwave degradation product, named as PC2, of lambda-carrageenan, was separated into three fractions by DEAE-cellulose DE-52 column chromatography. T-lymphocytes and macrophage obtained from healthy mice were cultured in the presence of the three polysaccharide fractions to evaluate their immunocompetence through cell proliferation, neutral red phagocytosis and nitric oxide（NO） release tests. The results indicated that the contents of sulfate and molecular weights of carrageenan fraction CF1, CF2 and CF3 increased in turn. All these fractions could significantly promote Con A caused spleen T-lymphocyte proliferation and also increase phagocytic activity of mouse peritoneal macrophage and NO release. In conclusion, the degraded lambda-carrageenan is able to enhance the immunity of healthy mice.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第11期232-236,共5页
Food Science
基金
山东省中青年科学家科研奖励基金项目(2008BS06012)
烟台市科学技术发展计划项目(2008GGA0020080410084458)
关键词
多糖
Λ-卡拉胶
降解组分
体外免疫活性
polysaccharide
lambda-carrageenan
degraded fractions
immunocompetence in vitro
