摘要
目的观察芍药苷对新生大鼠缺氧缺血性脑损伤(H IBD)中脑细胞凋亡的影响,并探讨其脑保护的作用机制。方法30只7日龄SD大鼠,随机均分成假手术组、缺氧缺血模型组、芍药苷低、中、高剂量治疗组。通过结扎左颈总动脉和吸入8%低氧混合气制备缺氧缺血性脑损伤(H IBD)动物模型。假手术组不进行缺氧缺血处理,各组新生鼠均于缺氧缺血后48 h处死,取大脑皮层及海马区组织进行HE染色及TUNEL法凋亡细胞检测。结果假手术组大鼠的大脑皮层及海马区组织、细胞形态正常;缺氧缺血模型组的大脑皮层及海马区组织水肿,细胞周围间隙增宽,胞体肿胀变圆、变大、胞浆染色变浅;各芍药苷组的神经元细胞胞体肿胀及组织水肿情况较缺氧缺血模型组明显好转。假手术组的大脑皮层及海马CA1区未见凋亡细胞;缺氧缺血模型组的可见较多凋亡细胞;各芍药苷组的凋亡细胞较相应时点缺氧缺血模型组的减少,差异有统计学意义(P<0.05)。结论芍药苷能减轻新生大鼠H IBD时的脑病理损伤,抑制大鼠H IBD时的脑细胞凋亡,对新生大鼠H IBD脑损伤具保护作用。
OBJECTIVE To study the effect of paeoniflorin（PF） on nerve cell apoptosis in neonatal rats with hypoxic-ischemic brain damage（HIBD） and investigate the neuroprotective effect of paeoniflorin against HIBD in neonatal rats.METHODS Thirty seven-day-old Sprague-Dawley（SD） rat pups were sham-operated and treated with vehicle（normal sodium injection）（Sham＋Veh） or hypoxia-ischemia（HI） and treated with either vehicle（HIBD＋Veh）,or PF 5 mg·kg^-1·d^-1（HIBD＋PF 5 mg·kg^-1·d^-1）,PF 10 mg·kg^-1· d^-1（HIBD＋PF 10 mg·kg^-1· d^-1）,PF 20 mg·kg^-1· d^-1（HIBD＋PF 20 mg·Kg-1· d^-1） by intraperitoneal injection 0h,24h after HI.HIBD rat models were established by ligating the left common carotid artery,followed by exposure to 8% oxygen and 92% nitrogen for 2 hrs in the HIBD＋Veh and the HIBD＋PF.The Sham＋Veh was not subjected to HI.After 48 hrs of HI,all the rats were sacrificed and then the brain tissues were removed.Apoptosis was examined by means of terminal-deoxynucleotidyl transferase Hediated d-UTP nick end labeling（TUNEL） and the positive cells were counted and the average value was calculated.Ematoxylin-eosin staining was exerted to observe the pathological changes of neonatal rats brain.RESULTS No TUNEL positive cells were observed in the cerebral cortex and hippocampal gyrum CA1 areas in the sham＋Veh.In the HIBD＋Veh,the apoptotic cells in the cerebral cortex and hippocampal gyrum CA1 areas were increased,but was significantly decreased in the HIBD＋PF group with the dose effect relation.Pathological changes showed that the number and structure of neurons in cortex and hippocampus gyrum CA1 were normal in the Sham＋Veh.Nevertheless,typical pathological changes following hypoxic ischemia（HI） were shown in cortex and hippocampus gyrum CA1 in the HIBD＋Veh,and these pathological changes were ameliorated by paeoniflorin treating.CONCLUSIONS Paeoniflorin could offer neuroprotection against HIBD and is possibly mediated by an anti-apoptotic effect in neona
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第4期354-356,共3页
West China Journal of Pharmaceutical Sciences
关键词
缺氧缺血
芍药苷
细胞凋亡
新生大鼠
Hypoxia-Ischemia
Paeoniflorin
Apoptosis
Neonatal rats
