摘要
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。
The antifungal activities of berberine and chitosan were studied by growth rate method. It was found that berberine of low concentration （0. 234 mg/mL） could inhibit the mycelial growth of vegetable pathogenic fungi, including Alternaria solani , Vermicularia capsici , Botrytis cinerea , Fusarium oxysporum f. sp. cucumerium, and Fusarium oxysporam f. sp. melonis. Another natural compound, ehitosan also inhibited their mycelial growth. Chitosan showed approximately 65% inhibition rate against B. cinerea. To integrate the two naturally occurring compounds, berberine-chitosan composite membrane was thus prepared and in vitro experiment revealed the berberine slow release, suggesting its potential utilization in controlling vegetable storage diseases.
出处
《北方园艺》
CAS
北大核心
2009年第8期131-133,共3页
Northern Horticulture
基金
北京市科协金桥工程资助项目
关键词
小檗碱
壳聚糖
复合膜
蔬菜病原真菌
Berberine
Chitosan
Composite membrane
Vegetable pathogenic fungi
