摘要
【目的】以北京棒杆菌(Coryne bacterium pekinense)1为研究对象,选育赖氨酸高产菌株,并探索赖氨酸产生菌基因组重排育种的基本规律。【方法】利用基因组重排技术选育赖氨酸高产菌株。【结果】通过四轮基因组重排成功选育出了5株遗传稳定的高产赖氨酸菌株,其中1株重排菌株赖氨酸产量达到16.95g/dL,比原始菌株Corynebacterium pekinense1赖氨酸产量提高了37.14%,比亲本菌株赖氨酸产量提高了17.46%～31.19%。【结论】首次采用基因组重排技术改良赖氨酸产生菌,成功选育出了5株产量较稳定的高产赖氨酸菌株,具有潜在的应用价值。
[Objective] To screen and breed high lysine-producing strains by genome shuffling. [Methods] Corynebacterium pekinense 1 was used as the starting strain in this study. High lysine-producing strains were screened by genome shuffling. [ Results] Five hereditarily stable strains with high lysine production were obtained by four cycles of genome shuffling. A high lysine producing strain, F4-36, was obtained, which produced 16.95 g/dL lysine, 37.1% higher than that of the starting strain. [ Conclusion] Genome shuffling can be an efficient tool to screen and breed high lysine production strains.
出处
《微生物学报》
CAS
CSCD
北大核心
2009年第8期1075-1080,共6页
Acta Microbiologica Sinica
基金
黑龙江省重大科技攻关项目(GA02C101)
黑龙江大学杰出青年科学基金项目资助~~
