摘要
目的:分析病毒和临床因素对HBeAg血清学状态和滴度的影响。方法:采用聚合酶链式反应联合限制性片段长度多态性方法,检测302例慢性乙型肝炎患者HBVDNA基因型及pC1896A和Bcp1762A/1764G突变,雅培方法检测HBeAg/HBeAb。结果:HBeAg(+)组HBVDNA(lgcopies/mL)为6.57(3.0～8.32)比HBeAg(-)组的4.76(3.0～8.75)高(P<0.001);HBeAg(+)组pC1896A变异发生率为33.3%,pC1896A和Bcp1762A/1764G两特定位点双变异发生率为10.9%,分别比HBeAg(-)组相应的62.3%、29.9%都低(均P<0.001)。通过多元线性逐步回归分析,发现HBVDNA载量和pC1896A变异是两个独立影响HBeAg滴度的因素。结论:HBVDNA载量是HBeAg滴度的正影响因素,pC1896A变异是其负影响因素。
Objective To find factors affecting HBeAg titer and serological status. Method HBV pC 1896A, Bcp 1762A/1764G and genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in a cohort of 302 patients with chronic hepatitis B; and HBsAg （s/n）, HBeAg （s/co）, and HBeAb （s/n） titer were detected by Abbott Achitect assay. Results HBV DNA （lg copies/mL） in HBeAg （＋） group [6.57 （3.0 - 8.32）] was higher than that in HBeAg （-） group [4.76 （3.0 - 8.75）] （P 〈 0.001）. The incidences of pC1896A mutation （33.3%） and of both pC1896A and Bcp1762A/1764G mutations （10.9%） in HBeAg （＋） group were lower than those in HBeAg （-） group （62.3% and 29.9%） （P 〈 0.001）. HBV DNA load and pC 1896A mutation were two independent factors affecting HBeAg titer found by multivariate linear gradual regressive analysis. Conclusion HBV DNA load is a positive factor on HBeAg titer, and pC1896A mutation is a negative factor.
出处
《实用医学杂志》
CAS
北大核心
2009年第13期2062-2064,共3页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:30500434)
