摘要
目的:定量检测慢性HBV感染患者血清中HBV-DNA与HBsAg、HBeAg,并寻找三者浓度之间的关系。方法:选取555例HBsAg阳性的慢性HBV感染患者血清标本,分别用荧光定量聚合酶链式反应法(FQ-PCR)检测HBV-DNA和微粒子酶免疫分析法(MEIA)检测HBsAg、HBeAg。结果:555例患者中,有338例检测出HBV-DNA阳性,阳性率为60.9%(338/555);对HBV-DNA浓度取对数后分为三组:>6组、3～6组和<3组。三组之间的HBsAg的浓度差异存在显著性(P<0.001),进一步两两比较发现>6组中位浓度低于3～6组(P<0.001),而3～6组和<3组之间差异无显著性(P=0.505);三组之间HBeAg差异有显著性(P<0.001),进一步进行两两比较,发现>6组高于3～6组(P<0.001),而3～6组高于<3组(P<0.001)。在HBV-DNA阳性组,对HBV-DNA浓度的对数与HBeAg进行相关分析,两者相关系数为0.463,而与HBsAg浓度呈负相关,相关系数为-0.180。结论:随着HBV-DNA拷贝数的增加,HBsAg量呈下降趋势,而HBeAg浓度呈上升趋势,且均存在相关性。
Objective To investigate the correlations of serum HBV-DNA, HBsAg, and HBeAg in patients with chronic hepatitis B （CHB）. Methods The serum samples of 555 patients with CHB were collected. HBV-DNA was tested by fluorescence quantitative polymerase chain reaction （FQ-PCR）, HBeAg and HBsAg were detected by microparticle enzyme immunoassays （MEIA）. Results Of the 555 CHD patients, 338 patients （60.9%） were HBV-DNA positive. The concentration of HBV-DNA was processed with logarithm （log HBV-DNA）, and according to which, patients were divided into 3 groups （ 〉 6 group, 3-6 group, 〈 3 group）. There was significant different of HBsAg and HBeAg among the 3 groups （P 〈 0.001 ）, and the further paired comparison suggested that HBsAg in 〉 6 group was significant lower than that in 3-6 group （P 〈 0.001 ）, but no significant difference of HBsAg was observed between 3-6 group and 〈 3 group （P = 0.505）; and HBeAg in 〉 6 group was significant higher than that in 3-6 group （P 〈 0.001 ）, which was significantly higher than that in 〈 3 group （P 〈 0.001）. In the HBV-DNA positive group, the correlation coefficient was 0.463 between HBeAg and log HBV-DNA, and was -0.180 between HBsAg and log HBV-DNA. Conclusion With the increase of HBV-DNA, HBeAg was increased, but HBsAg was decreased.
出处
《实用医学杂志》
CAS
北大核心
2010年第19期3627-3629,共3页
The Journal of Practical Medicine
基金
江苏省实验诊断学重点实验室基金(编号:XK200731)
