摘要
以1a生北丰(Vitis thunbergii×V.vinifera)葡萄盆栽结果树为试材,在果实始熟期采用紫外线C(Ultraviolet C,UV-C)辐射结合韧皮部环剥的方法,研究了UV-C辐射对葡萄叶片和邻近果穗果实白藜芦醇(Resveratrol,Res)及其糖苷质量分数的影响。结果表明,UV-C辐射诱导葡萄果实Res合成积累的敏感性低于叶片,辐射葡萄叶片对邻近果穗上果皮Res的影响远远小于UV-C直接辐射葡萄果穗,且辐射诱导葡萄果皮中Res质量分数与叶片中Res质量分数存在着一定的相关性。当叶片位于果穗下方时,叶片中反式白藜芦醇(trans-Resveratrol,trans-Res)、反式白藜芦醇苷(trans-Piceid,trans-PD)和顺式白藜芦醇苷(cis-Piceid,cis-PD)质量分数表现出环剥处理显著高于不环剥处理,然而果皮中的结果正好相反。当叶片位于果穗上方时,果皮中trans-Res、trans-PD和cis-PD质量分数表现出不环剥处理显著高于环剥处理,暗示葡萄Res可能存在韧皮部运输途径,且运输方向是双向的。此外,UV-C直接辐射葡萄果穗处理果皮trans-Res和cis-PD质量分数显著高于对照和UV-C辐射葡萄叶片处理。种子中仅检测到trans-Res,且仅环剥处理和辐射下方叶片不环剥处理显著高于对照。
One-year-old Beifeng grape （ Vitis thunbergii×V. vinifera） potted fruited vines were used as materials to study the effects of UV-C irradiation on resveratrol （Res） and its glycosides concent in grape leaves and berries of neighboring clusters with the method of UV-C irradiation combined with phloem girdling at veraison. The results showed： the sensitivity in UV-C irradiation-induced Res accumulation in berries was lower than that in leaves. There was certain pertinence in UV-C-induced Res content in berry skin and those of grape leaves. When UV-C irradiated leaves located beneath fruit clusters, trans-Res,trans-PD and cis-PD content in grape leaves of girdling treatment （＊Ldown）were significantly higher than those of non- girdling treatment （Ldown）, however, the results in berry skin were just the opposite. When UV-C irradiated leaves located higher than fruit clusters,trans-Res,trans-PD and cis-PD content in grape berry skin of non-girdling treatment （Lup） were significantly higher than those of girdling treatment （＊ Lup）. The results indicated that there might be phloem transport chan- nel of grapes Res and the transport was two-way directions. In addition ,trans-Res and cis-PD in grape berry skin of UV-C irradiated fruit clusters treatment （Fuv） were significantly higher than those of control and UV-C irradiated leaves treatments. Only trans-Res was detected in grape seeds,and only the girdling treatments （＊Lup and ＊Ldown） and UV-C irradiated leaves located beneath fruit clusters and non-girdling treatment （Ldown） were significantly higher than controls.
出处
《果树学报》
CAS
CSCD
北大核心
2009年第4期461-465,共5页
Journal of Fruit Science
基金
国家自然科学基金(30800744)
中国科学院知识创新工程方向性项目(KSCX2-YW-N-032)
