摘要
探讨了增强里氏木霉ＲｕｔＣ－３０产纤维素酶的方法，添加葡糖糖于培养基中，可促进菌体生长，但不能提高产酶；采用Ａｖｉｃｅｌ与麸皮复合碳源，以及使用ＫＨ２ＰＯ４－Ｋ２ＨＰＯ４缓冲系统控制发酵液ｐＨ，在摇瓶发酵条件下，可获得很高活力的纤维素酶，培养６ｄ，酶活可达到ＣＭＣａｓｅ１６６７～２０８４ｎｍｏｌ·ｓ－１·ｍｌ－１，ＦＰＡ１５０～２００ｎｍｏｌ·ｓ－１·ｍｌ－１．采用２．５Ｌ发酵罐培养，通过控制ｐＨ和溶氧，纤维素酶活力为ＣＭＣａｓｅ２２２３．８ｎｍｏｌ·ｓ－１·ｍｌ－１，ＦＰＡ１９４．５ｎｍｏｌ·ｓ－１·ｍｌ－１．
This paper discussed enhancement in cellulase production by Trichoderma reesei Rut C30. It was found that glucose can promote cell growth, but it didn't increase cellulase production. A combination of Avicel and wheat bran was used as carbon source. The KH2PO4K2HPO4 buffer system was used for pH control, resulting in very high enzyme production in shake flask culture, Maximum CMCase 1667￣2084 nmol·s-1·ml-1, FPA 150￣200 nmol·s-1·ml-1 were achieved within 6 days' culture. When it was grown in 2.5 L fermentor, where pH and DO levels were controlled, the enzyme activity were 2223.8 nmol·s-1·ml-1 of CMCase activities and 194.5 nmol·s-1·ml-1 of FP activities respectively.
基金
韩国贸易
工业
能源部基金
