摘要
为探明三峡库区岸生植物秋华柳(Salix varegata Franch)的水淹耐受机理,以1年生秋华柳实生苗叶片为实验材料,测定了处理时间分别为3、9、15和30 d时未水淹对照植株和水淹2 m植株的超氧阴离子和丙二醛(MDA)的含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。结果显示:水淹过程中,1)水淹2 m植株超氧阴离子含量显著上升,但在水淹9 d之前与对照相比无显著差异;2)水淹2 m植株SOD和POD活性显著上升,分别在水淹15、30 d时达到最大值,两者均与超氧阴离子含量呈显著正相关;3)水淹2 m植株MDA含量均高于对照,并在水淹15、30 d时差异显著,但不同水淹持续时间差异不显著。研究结果暗示出秋华柳通过提高酶活性来降低活性氧对细胞膜的伤害以适应水淹胁迫。
To investigate the submergence-tolerant mechanism of riparian plant Salix varegata Franch in Three Gorges Reservoir region, the leaves of one-year S. varegata seedlings as the material are selected and studied, under different submerged times （3, 9, 15 and 30 d）, the changes of superoxlde radica/, malondialdehyde （MDA） content and activities of protective enzymes in leaves of control group and 2m-submerged group （2 m beneath water surface） . The results show that during the submerged times, 1 ） The superoxide radical content of 2 m-submerged plants increases significantly, but has not a obvious change before the 9th day. 2） The SOD and POD activities of 2 m-submerged plants increase significantly, reaching the summit on the 15th and 30th day each. Both have positive correlation with superoxide radical content. 3） The MDA content of 2 m-submerged plants are higher than that of control, having significant differences on the 15th and 30th day, but no significant differences between different submergence-sustain-time groups. The results above indicate that S. varegata can survive in submerged environment by enhancing activities of protective enzymes and reducing damages brought by active oxygen radicals.
出处
《重庆师范大学学报（自然科学版）》
CAS
2009年第3期30-33,共4页
Journal of Chongqing Normal University:Natural Science
基金
国家自然科学基金(No.30770406
No.30500041
No.30440035)
教育部新世纪优秀人才支持计划项目(No.NCET-06-0773)
国家科技支持计划项目(No.2006BAC10B01)
重庆市科技攻关项目(No.CSTC2007AB049)
中国科学院西部行动计划项目(No.KZCX2XB2-07)
关键词
三峡库区
水淹
秋华柳
丙二醛(MDA)
保护酶
Three Gorges Reservoir Region
submergence
Salix varegata Franch
malondialdehyde （MDA）
protective enzymes
