摘要
以药用观赏植物鸡冠花幼苗为材料,在17%PEG-6000模拟干旱胁迫条件下,用不同浓度NO供体硝普钠、甜菜碱、腐植酸处理幼苗,测定叶片的光合色素、丙二醛、可溶性糖、可溶性蛋白和超氧阴离子的含量和超氧化物歧化酶、过氧化物酶、过氧化氢酶活性.结果显示:各NO供体硝普钠、甜菜碱、腐植酸处理较对照均显著提高了干旱胁迫下鸡冠花幼苗叶片的光和色素、可溶性糖及可溶性蛋白的含量,却显著降低了鸡冠花叶片的丙二醛含量和超氧阴离子含量,同时显著提高了干旱胁迫下鸡冠花叶片中的超氧化物歧化酶,过氧化物酶和过氧化氢酶的活性,且以0.5mmol/L NO供体硝普钠、1.0g/L甜菜碱、200mg/L腐植酸处理后的酶活性最强.研究表明,适宜浓度的NO供体硝普钠(0.5mmol/L)、甜菜碱(1.0g/L)、腐植酸(200mg/L),通过调节渗透调节物质含量和保护酶活性来有效减缓干旱胁迫对鸡冠花幼苗的伤害,提高鸡冠花幼苗的抗旱能力.
Celosia cristataseedlings were cultured under drought stress simulated with 17%PEG-6000and treated with SNP（an exogenous nitric oxide donor）,betaine and humic acid at different concentrations.Then the contents of photosynthetic pigments,malondialdehyde（MDA）,soluble sugars,soluble protein and superoxide anion,and the activities of superoxide（SOD）,peroxidase（POD）and catalase（CAT）in the leaves were measured so as to develop a method to improve the drought resistance of this medical ornamental plant species.All treatments improved the contents of chlorophyll a,chlorophyll b,chlorophyll k,total chlorophyll,soluble sugars and proline and decreased the contents of MDA and superoxide anions under drought stress.They also increased the activities SOD,POD and CAT.The highest SOD,POD and CAT activities were recorded in the treatment of SNP,betaine and humic acid at 0.5mmol/L,1.0g/L and 200mg/L,respectively.In conclusion,SNP（0.5mmol/L）,betaine（1.0g/L）and humic acid（200mg/L）can significantly alleviate the damages to the seedlings of C.cristata under drought stress and promote their drought resistance.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期14-21,共8页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金项目(30070080)基金资助
关键词
鸡冠花
NO供体硝普钠(SNP)
甜菜碱
腐植酸
干旱胁迫
生理特性
Celosia cristata L.
exogenous nitric oxide donor SNP
drought stress
betaine
humic acid
physiological character
