摘要
通过正交实验,研究了由黑曲霉发酵生产壳聚糖的工艺条件,确定最佳培养基为玉米浆3%、葡萄糖3%、硫酸镁1.0%、磷酸二氢铵0.01%、磷酸二氢钾0.005%;最佳发酵条件为接种量10%、发酵时间90 h、摇床转速180 r.min-1、pH值6、发酵温度30℃。最佳工艺下的菌体干重达51.52 mg.mL-1,利用酸碱法提取壳聚糖,其脱乙酰度达78.09%。
The production process of chitosan by fermentation of Aspergillus niger was studied by orthogohal experiment. The optimum components of culture medium were determined as follows, corn steep liquor 3%, glucose 30%, magnesium sulfate 1.0% ,ammonium dihydric phosphate 0.01% ,potassium dihydrogen phosphate 0. 005%, and the optimum fermentation conditions were as follows, the fermentation time was 90 h,shaking speed was 180 r·min^-1 ,pH value was 6, the fermentation temperature was 30℃ and the inoculation amount was 10%. Under the optimum fermentation process,the dry cell weight could reach 51.52 mg · mL^-1. When chitosan was extracted by acid-base method,its deacetylation degree was 78.09%.
出处
《化学与生物工程》
CAS
2009年第6期86-87,共2页
Chemistry & Bioengineering
关键词
黑曲霉
提取
壳聚糖
发酵工艺
Aspergillus niger
extraction
chitosan
fermentation process
