摘要
目的:研究卵巢去势大鼠阴道平滑肌中兰尼碱受体1(RyR1)和L-型钙通道V1.3(Cav1.3)的表达,以探讨RyR1和Cav1.3与雌激素在女性性功能障碍中的相关性。方法:40只8周龄雌性SD大鼠随机均分成:假手术2周组(A组)、假手术4周组(B组)、去势2周组(C组)和去势4周组(D组)。术后2、4周运用全自动化学发光免疫法测定各组大鼠血清雌激素水平,RT-PCR和免疫组化法检测阴道平滑肌中RyR1和Cav1.3的mRNA及其蛋白表达。灰度比值表示RyR1和Cav1.3的mRNA表达量,积分光密度值表示RyR1和Cav1.3蛋白表达量。结果:血清雌激素水平C组[(0.210±0.026)nmol/L]较A组[(0.505±0.053)nmol/L]显著降低(P<0.01),D组[(0.130±0.031)nmol/L]较B组[(0.476±0.058)nmol/L]显著降低(P<0.01)。RyR1和Cav1.3在各组大鼠阴道平滑肌内均有表达。RyR1mRNA灰度比值C组(0.680±0.073)较A组(0.950±0.064)显著降低(P<0.01),D组(0.220±0.032)较B组(0.890±0.072)显著降低(P<0.01);Cav1.3mRNA灰度比值C组(0.580±0.043)较A组(0.870±0.019)显著降低(P<0.01),D组(0.190±0.020)较B组(0.820±0.021)显著降低(P<0.01)。RyR1蛋白表达积分光密度值C组(96.67±7.75)较A组(123.69±10.66)显著降低(P<0.01),D组(86.45±8.16)较B组(109.31±9.87)显著降低(P<0.01);Cav1.3蛋白表达积分光密度值C组(87.97±6.96)较A组(106.46±8.04)显著下降(P<0.01),D组(69.43±8.30)较B组(97.38±7.56)显著降低(P<0.01)。结论:RyR1和Cav1.3在大鼠阴道平滑肌内均有表达,雌激素可能通过影响大鼠阴道平滑肌中RyR1和Cav1.3的表达参与女性性反应调控。
Objective： To determine the expressions of ryanodine receptor type 1 （RyR1） and Cav1. 3 L-type calcium channel （Car1. 3） in the vaginal smooth muscle cells of castrated rats and investigate the correlation of RyR1 and Car1. 3 with estrogen in female sexual dysfunction. Methods： Forty female SD rats of 8 weeks were randomly divided into Croups A （2-week sham operation） , B （4-week sham operation） , C （2-week castration） and D （4-week castration）. Two and 4 weeks after surgery, the serum estradiol level was determined with the automated immunoehemiluminescence system and the expressions of RyR1 and Car1. 3 in the vaginal smooth muscle were detected by immunohistochemistry and RT-PCR. Gray scale ratio was used to represent the mRNA expression levels of RyR1 and Cav1. 3, and the optical density value to denote their protein expression levels. Results ： Serum estradiol was significantly decreased in Group C （ [ 0. 210 ±0. 026 ] nmol/L） as compared with A （ [ 0. 505 ±0. 053 ] nmol/L） （ P 〈 0. 01 ）, and so was it in Group D （[0.130 ± 0.031] nmol/L） in comparison with B （[0.476 ± 0.058] nmol/L） （P 〈 0.01）. RyR1 and Cav1. 3 were expressed in all groups. The mRNA expressions of RyR1 and Cav1. 3 were significantly reduced in Group C （0. 680 ± 0.073 and 0.580 ± 0.043） as compared with A （0.950 ± 0.064 and 0.870 ± 0.019） （P 〈 0.01）, as well as in Group D （0. 220 ± 0. 032 and 0. 190 ± 0. 020） in comparison with B （0. 890 ± 0. 072 and 0. 820 ± 0. 021 ） （ P 〈 0.01 ）. The protein expressions of RyR1 and Car1. 3 were significantly down-regulated in Group C （96.67 ± 7.75 and 87.97 ± 6.96） as compared with A （ 123.69 ± 10.66 and 106.46 ± 8.04） （P 〈 0.01 ）, and so were they in D （86.45 ± 8.16 and 69.43 ± 8.30） in comparison with B （109.31 ± 9.87 and 97.38 ± 7.56） （P 〈 0.01 ）. Conclusion： Both RyR1 and Cav1. 3 were expressed in the vaginal smooth muscle ceils of the rats, and estrogen might be involve
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第6期511-516,共6页
National Journal of Andrology
基金
四川省卫生厅基金(060045)
四川省教育厅基金(2005B017)
四川省科技厅基金(2006J13-146)~~
