摘要
目的:研究兰尼碱受体1(RyR1)和电压门控钙离子通道1.3(CaV1.3)在去势大鼠阴茎海绵体平滑肌的表达,探讨其在去势后勃起功能障碍发生中的作用。方法:40只8周龄雄性SD大鼠随机均分成:假手术2周组(A组),假手术4周组(B组),去势2周组(C组),去势4周组(D组)。术后实验各组检测血清睾酮(T)水平,免疫组化及RT-PCR技术检测RyR1和CaV1.3在大鼠阴茎海绵体平滑肌中的表达。结果:血清T水平C组[(15.97±5.67)nmol/L]和D组[(2.03±1.57)nmol/L]分别较A组[(90.54±20.13)nmol/L]和B组[(120.35±30.32)nmol/L]显著下降(P<0.05)。RyR1、CaV1.3在各组大鼠阴茎海绵体平滑肌中均有表达,RyR1 mRNA相对表达量灰度比值C组(0.51±0.24)和D组(0.33±0.15)分别较A组(1.53±0.25)和B组(1.37±0.23)显著降低(P<0.05);CaV1.3 mRNA相对表达量灰度比值C组(0.50±0.12)和D组(0.32±0.07)分别较A组(1.33±0.05)和B组(1.25±0.03)显著降低(P<0.05)。RyR1蛋白积分光密度值(IA)C组(120.36±25.78)和D组(67.39±30.54)分别较A组(300.96±135.12)和B组(330.38±128.59)显著降低(P<0.05);CaV1.3蛋白IA C组(103.37±39.52)和D组(67.56±20.15)分别较A组(298.68±126.35)和B组(327.35±117.37)显著降低(P<0.05)。雄激素水平与RyR1、CaV1.3在阴茎海绵体平滑肌中的表达水平具有正相关性。结论:雄激素可能通过RyR1、CaV1.3的表达调控阴茎勃起功能。
Objective： To study the expressions of ryanodine receptor 1（RyR1） and voltage-gated calcium channel 1.3（CaV1.3） in the corpus cavernosum smooth muscle of castrated rats and to investigate their role in androgen deficiency-related erectile dysfunction.Methods： Forty 8-week-old SD rats were equally randomized into Groups A（2-week sham-operation）,B（4-week sham-operation）,C（2-week castration）,and D（4-week castration）.After surgery,the levels of serum testosterone in different groups of rats were determined,and the expressions of RyR1 and CaV1.3 in the corpus cavernosum were detected by immunohistochemical staining and RT-PCR.Results： The levels of serum testosterone were significantly decreased in Groups C（[15.97 ± 5.67] nmol/L） and D（[2.03 ± 1.57] nmol/L） as compared with A（[90.54 ± 20.13] nmol/L） and B（[120.35 ± 30.32] nmol/L）（P 0.05）.RyR1 and CaV1.3 expressed in all the groups.RyR1 mRNA,CaV1.3 mRNA and their proteins were remarkably reduced in Groups C（0.51 ± 0.24,0.50 ± 0.12,120.36 ±25.78,103.37 ± 39.52,respectively） and D（0.33 ± 0.15,0.32 ±0.07,67.39 ± 30.54,67.56 ±20.12,respectively） in comparison with A（1.53 ± 0.25,1.33 ± 0.05,300.96 ± 135.12,298.68 ± 126.35,respectively） and B（1.37 ± 0.23,1.25 ± 0.03,330.38 ± 128.59,327.35 ±117.37,respectively）（P 0.05）.The androgen level was positively correlated with the expressions of RyR1 and CaV1.3.Conclusion： Androgen can regulate erectile function via RyR1 and CaV1.3.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第10期895-900,共6页
National Journal of Andrology
基金
四川省教育厅研究基金(2005B017)
四川省卫生厅研究基金(060045)
四川省科技厅研究基金(2006J13-146)~~
