摘要
目的建立诺如病毒(Norovirus,NoV)结合唾液HBGAs受体的实验方法、验证2株GⅡ-4型NoV毒株与我国人群唾液HBGAs的结合方式。方法收集健康志愿者唾液标本,采用凝集抑制实验法检测唾液中HBGAs血型物质,用EIA法检测NoV VLPs、阳性毒株与HBGAs的结合情况。结果63份标本检出O型21例(占33.3%),B型14例(占22.2%),A型和非分泌型各13例(分别占20.6%),AB型2例(占3.2%)。rVA387、2株GⅡ-4型毒株均能结合分泌型,与非分泌型唾液不反应。结论本研究成功建立了NoV结合唾液HBGAs受体的实验方法,通过NoV毒株与HBGAs相互作用的研究有助于了解NoV的宿主适应特性及病毒进化和流行规律;同时为筛选防治我国人群的抗NoV药物提供技术平台。
To establish an experimental method for the study on the combination of Norovirus （NOV） GⅡ type with the human blood group antigen （HBGA） receptor in saliva of Chinese populations, saliva specimens were collected from 63 volunteers and the agglutination inhibition test was used to identify the types of HBGAs. Meanwhile, the EIA assay was employed to analyze the binding patterns of NoV GⅡ to HBGAs. It was found that among these 63 volunteers, O-group antigen ,B-group antigen, A and non-secretor and AB-group antigen were accounted for 33.3% ; 22.2%, 20.6% and 3.2% respectively. Recombinant VA387 virus-like particles （rVA387） and 2 strains of NoV GⅡ could bind with HBGAs of secretors, but not to ali the non-secretors. It is concluded from this study that the establishment of a experimental method for the study on the combination of norovirus with human blood group antigens may help us to understand the adaptability of norovirus in the host and the evolutionary as well as epidemiologic rules about this viral infection.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2009年第6期560-562,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助课题(No30572458
No30700716)
