摘要
目的了解广州市5岁以下婴幼儿诺如病毒感染状况及对诺如病毒阳性株进行基因型分析。方法收集广州市2008年5家病毒性腹泻监测点医院的门诊临床诊断为病毒性腹泻的5岁以下患儿临床资料及粪便标本692份,采用ELISA方法检测轮状病毒、腺病毒、星状病毒和诺如病毒抗原,将诺如病毒抗原阳性的标本用RT-PCR方法进行诺如病毒核酸扩增,阳性产物回收、纯化并测序,结合GenBank中的相关序列进行型别流行特点分析。结果 692份标本中,ELISA方法检测4种病毒的阳性率分别为轮状病毒31.9%(221/692)、诺如病毒21.2%(147/692)、腺病毒6.1%(42/692)、星状病毒1.2%(8/692),混合感染占6.8%(47/692)。诺如病毒腹泻病例发病高峰在9—11月份,占51.7%(76/147),2岁以下病例数占91.8%(135/147)。临床症状以腹泻、呕吐、发热、腹痛为主。147份诺如病毒ELISA阳性标本中RT-PCR方法检测诺如病毒核酸阳性率为84.4%(124/147),经测序及与GenBank中的相关序列比对,证实124份均属诺如病毒GⅡ遗传组,其中1份是GⅡ2型,其余均为GⅡ4型,GⅡ4型中又有不同分支。结论诺如病毒是广州市5岁以下儿童病毒性腹泻的常见病原之一,仅次于轮状病毒,常见于2岁以下婴幼儿,流行优势株基因型为GⅡ4型。
Objective To describe epidemiologic characteristics of novovirus infection and its genotype in children under 5 years in Guangzhou.Methods Stool specimens and clinical data were collected from 692 outpatients with clinically-diagnosed viral diarrhea from five surveillance hospitals in 2008.Specimens were tested for antigens of rotavirus,adenovirus,norovirus,and astrovirus by ELISA,and those positive for norovirus were subject to reverse transcription polymerase chain reaction（PCR）.Positive PCR products were purified and sequenced,and the sequences were compared to GenBank data to identify genotype.Results Of all 692 specimens,31.9%（221）,21.2%（147）,6.1%（42）,and 1.2%（8） were positive for antigens of rotavirus,norovirus,adenovirus,and astrovirus,respectively.Mixed infection accounted for 6.8%（47/692） of all cases.For norovirus cases,there was a peak of occurrence during September to November,which corresponded to 51.7%（76/147） of all cases;children under 2 years accounted for 91.8%（135/147） of all cases;main clinical symptoms were diarrhea,vomiting,fever,and abdominal pain.Nucleic acid of norovirus was detected in 84.4%（124/147） of the specimens positive for norovirus antigen,and all strains belonged to genotype GⅡ4,except for one（GⅡ2）.Conclusion Norovirus is only second to rotavirus in the frequency of causing viral diarrhea in children under 5 years of age in Guangzhou.Norovirus infection is clinically common in infants under 2 years old,and GII4 is the predominant genotype in the population studied.
出处
《华南预防医学》
2010年第5期1-4,10,共5页
South China Journal of Preventive Medicine
基金
广州市医药卫生科技项目(2007-YB-124)
广州市医药卫生科技项目重点项目(2008-ZDi-13)
